Msc-macrophage Co-culture
Msc-macrophage Co-culture - Related Videos
04:46
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11:05
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Cited by 21 •
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The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
06:46
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Cited by 58 •
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A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
03:05
In Vitro Culture and Differentiation of Primary Monocytes into Macrophages
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2025
This video demonstrates the priming of primary monocytes into macrophages. Primary human monocytes can be isolated and differentiated to an M1-like pro-inflammatory phenotype using the granulocyte-macrophage colony-stimulating factor (GM-CSF) or to an M2-like anti-inflammatory phenotype using the macrophage colony-stimulating factor (M-CSF).
11:13
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
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Cited by 47 •
2017
Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.
FAQs
The two cell types can communicate through physical contact and through soluble factors released into the shared culture environment. These routes may act together or produce distinguishable effects on macrophage activation and inflammatory mediator production. Comparing responses in a shared culture helps investigators examine how MSC signals influence macrophage behavior beyond the effects of either cell type studied separately.
Macrophage polarization describes shifts in macrophage functional state in response to environmental cues. MSC-derived signals can modify this state, making polarization a useful readout of immune regulation within the culture. Examining polarization alongside activation and mediator production helps connect cellular communication with broader changes in inflammatory behavior, particularly when modeling conditions relevant to infection or tissue repair.
Environmental cues determine how macrophages respond to signals from MSCs and can change activation, inflammatory mediator production, and polarization. This makes the surrounding experimental context important when interpreting results: the same MSC-macrophage interaction may produce different immune outcomes under different cues. Such comparisons help separate general regulatory effects from responses associated with particular inflammatory or infectious settings.
Changes in inflammatory mediator production provide evidence that MSC-macrophage communication has altered immune activity. When interpreted together with macrophage activation and polarization, these measurements can indicate whether the interaction is associated with inflammatory regulation rather than an isolated molecular change. The resulting profile helps researchers investigate mechanisms connecting MSC activity, macrophage function, and host responses.
In infection research, the model provides a controlled setting for examining how MSC signals influence macrophage responses to pathogen-related environmental cues. Researchers can assess resulting changes in macrophage activation, inflammatory mediators, and polarization to characterize immune regulation during a host response. This approach connects cellular communication with questions about how inflammation is shaped during infection.
A shared culture allows investigators to examine the effects of MSCs on macrophage function under defined experimental conditions. Measurements of activation, inflammatory mediator production, and polarization can reveal how an intervention changes immune behavior. The system is also useful for studying links between immune regulation and tissue repair, providing mechanistic context for MSC-based strategies without relying only on whole-organism outcomes.