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MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
JoVE Journal
Biology
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JoVE Journal Biology
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
DOI:

11:13 min

February 22, 2017

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Chapters

  • 00:05Title
  • 01:09Seeding of Glioblastoma Stem Cells
  • 03:00Mesenchymal Stem Cells Mitochondria Isolation
  • 06:13Transfer of Isolated MSC Mitochondria to GSCs
  • 07:50Results: Isolation and Transfer of Mitochondria into Mesenchymal Stem Cells
  • 09:39Conclusion

Summary

Automatic Translation

Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.

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