MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

Brice Nzigou Mombo; Sabine Gerbal-Chaloin; Aleksandra Bokus; Martine Daujat-Chavanieu; Christian Jorgensen; Jean-Philippe Hugnot; Marie-Luce Vignais

doi:10.3791/55245

Journal

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Biology

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MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

JoVE Journal
Biology

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JoVE Journal Biology

MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

12,818 Views

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11:13 min

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February 22, 2017

DOI:

10.3791/55245-v

DOI:

10.3791/55245-v

•

11:13 min

February 22, 2017

•

12801 Views

Brice Nzigou Mombo¹, Sabine Gerbal-Chaloin¹, Aleksandra Bokus¹, Martine Daujat-Chavanieu¹, Christian Jorgensen¹, Jean-Philippe Hugnot², Marie-Luce Vignais¹

¹Institute for Regenerative Medicine and Biotherapy (IRMB), INSERM U1183,Montpellier University ²Institute of Neurosciences of Montpellier (INM), INSERM U1051,Montpellier University

Summary

Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.