Nasal Lavage
Nasal Lavage - Related Videos
05:12
Murine Nasal Lavage Fluid Collection without Blood Contamination
0 Views •
2025
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory pathogens.
04:35
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
0 Views •
2024
Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
11:47
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
0 Views •
Cited by 47 •
2014
We describe a research technique for fiberoptic bronchoscopy and bronchoalveolar lavage using low pressure suction. The technique is used to harvest immune cells from the lung bronchoalveolar surfaces. Local anesthetic and mild conscious sedation (midazolam) is used. Subjects tolerate the procedure well and experience minimal side effects.
07:03
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
0 Views •
Cited by 155 •
2017
The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
02:17
Bronchoalveolar Lavage Fluid Collection from a Mouse Model Infected with Non-typeable Haemophilus influenzae
0 Views •
2025
Source: Venuprasad, K., et al. Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (2016)This video demonstrates the collection and processing of bronchoalveolar lavage fluid from a euthanized mouse infected with non-typeable Haemophilus influenzae for downstream analysis.
FAQs
Nasal lavage can recover mucus-associated cells, proteins, allergens, and microbial components along with loosened secretions. These materials provide different readouts: cells and proteins can support assessment of local inflammation and immune mediators, while microbial components can support pathogen-related investigation. Examining these fractions together helps connect mucosal immune activity with events occurring in the upper airway.
Sterile saline provides fluid that loosens material adhering within the nasal passages. As secretions become suspended, the rinse can transport them into a collection vessel rather than leaving them confined to the cavity. This improves access to cellular, protein, allergen, and microbial material that would otherwise be difficult to analyze directly.
Its value comes from sampling the airway surface, where local inflammation, immune mediators, allergens, and microbial material may be present. The collected fluid therefore gives researchers a relatively noninvasive view of airway biology, supporting studies of mucosal defense and allergic or infectious responses in the upper airway.
A basic workflow introduces sterile saline into the nasal cavity, allows it to loosen secretions, and directs the resulting fluid into a collection vessel. The sample can then be examined for suspended cells, proteins, allergens, and microbial components, depending on the research question. This links the physical rinse to downstream immunology or infection analyses.
Beyond detecting pathogens, researchers can use collected material to investigate allergic disease and mucosal defense. Allergen content can help characterize what is present in the airway, while cellular and protein measurements can support assessment of local inflammatory or immune activity. The same sampling approach therefore connects allergen presence with airway immune responses.
In infection and vaccination studies, nasal lavage can help examine airway-associated findings across these biological contexts. Measurements may include local inflammatory signals, immune mediators, cells, or microbial components recovered from the rinse. This focus is important when research asks how the upper-airway mucosa participates in defense, rather than examining pathogen presence alone.