Parafilm Gasket
Parafilm Gasket - Related Videos
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
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2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
08:21
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
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Cited by 5 •
2016
We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.
10:59
Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
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Cited by 8 •
2010
To study Myxococcus xanthus swarm behavior, we have designed a time-lapse microcinematography protocol that can be modified for different assays. It employs standard growth conditions adapted for microscopy, and yields reproducible results by the use of inexpensive, reusable silicone gaskets. We have used this method to quantify multicellular chemotaxis.
02:56
Ultrasound-Induced Neuromodulation Using a Microelectrode Array System
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2025
This video demonstrates the method for inducing the modulation of neural activity in cultured human iPSC-derived neurons in a multi-well microelectrode array, or MEA system using a focused ultrasound transducer.
09:03
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
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Cited by 23 •
2013
Reconstituting functional membrane proteins into giant liposomes of defined composition is a powerful approach when combined with patch-clamp electrophysiology. However, conventional giant liposome production may be incompatible with protein stability. We describe protocols for producing giant liposomes from pure lipids or small liposomes containing ion channels.
FAQs
Stretching allows the film to conform to small irregularities around an opening, while compression between contacting surfaces helps maintain contact at the junction. This conformity reduces pathways through which liquid may escape or airborne contaminants may enter. The result is a temporary barrier that supports more stable conditions during incubation, transport, or storage of laboratory materials.
A Parafilm gasket can help limit evaporation, spills, and entry of airborne contaminants, all of which may disturb an experiment. These effects are especially relevant when samples or reagents remain exposed during incubation, transport, or storage. The film supports containment and condition control, but the overview does not establish it as a validated barrier for every experimental environment.
The main distinction is the level of assurance provided. A Parafilm gasket offers a low-cost, adaptable, and easily removable temporary barrier, whereas a validated sterile closure is intended to meet established containment or sterility requirements. Consequently, the film may support routine handling of immunology and infection materials, but it should not be treated as a replacement for validated closure systems.
Conformability allows the film to accommodate small irregularities where laboratory components meet. This matters because a junction does not always present perfectly uniform contacting surfaces. By adapting to those minor variations, the gasket can help reduce gaps associated with evaporation, spills, or airborne entry. Its usefulness therefore depends on maintaining contact across the specific container, lid, plate, or component junction.
Position the film across the opening or junction, then stretch it over the exposed area or compress it between the contacting components. The material should remain in contact with the surfaces it is intended to separate from the surrounding environment. After the incubation, transport, or storage period, its easy removal allows access without establishing a permanent closure.
Within immunology and infection workflows, the film may be useful for microplates, cultures, and diagnostic assays when samples or reagents need added protection during incubation, transport, or storage. It can help maintain sample stability by limiting evaporation, spills, and airborne contamination. Its role is supportive containment, not replacement of validated sterile closures or other required controls.