Peritoneal Leukocytes
Peritoneal Leukocytes - Related Videos
05:12
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
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Cited by 11 •
2015
This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.
07:06
Selective Harvesting of Marginating-pulmonary Leukocytes
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Cited by 1 •
2016
Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases.
03:42
Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
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2025
In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.
07:51
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Cited by 44 •
2015
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
FAQs
Chemokines and inflammatory cytokines help determine which leukocytes enter the peritoneal cavity and when. These signals promote movement of circulating monocytes and neutrophils across the mesothelial barrier, adding rapidly recruited cells to resident macrophages and lymphocytes. The resulting shift links local sensing with microbial elimination and removal of damaged material during infection, injury, or inflammation.
Resident macrophages and lymphocytes provide ongoing surveillance, whereas recruited monocytes and neutrophils appear in response to inflammatory signals. This distinction matters because cell origin and timing can indicate whether the cavity is in a monitoring state or responding to a developing challenge. Comparing these populations helps separate baseline immune organization from an induced inflammatory response.
The balance between resident and recruited cells can change as local conditions change. Chemokines and inflammatory cytokines provide directional signals, while the mesothelial barrier regulates passage from circulation into the cavity. Together, these mechanisms determine whether monocytes and neutrophils are added to the resident macrophage and lymphocyte population, shaping the intensity and composition of the local response.
Analysis should distinguish resident macrophages and lymphocytes from circulating monocytes and neutrophils recruited into the cavity. This comparison reveals whether observed cells represent the local surveillance population or an inflammatory influx. Relating the composition to infection, injury, or inflammation helps investigators interpret changes in host defense and immune regulation rather than treating all leukocytes as one group.
It can show how local immune populations respond when microbes challenge the abdominal cavity, including the contribution of resident cells and the recruitment of circulating cells. Such measurements support investigation of microbial elimination, removal of damaged material, and changes in inflammatory regulation. They also provide a basis for studying therapies designed to alter leukocyte recruitment or function.
Because these cells operate at a site where infection, injury, and inflammation can provoke distinct local responses, their composition and activity offer a way to study abdominal inflammatory disease. Researchers can use population changes to connect immune surveillance, leukocyte recruitment, and removal of damaged material with disease mechanisms or treatments intended to modify local immunity.