Radiolabeled Nucleotides
Radiolabeled Nucleotides - Related Videos
03:35
Assessing Viral Replication Capacity Using a Radiolabeled Reverse Transcriptase Assay
0 Views •
2026
Source: Claiborne, D. T. et al. A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses. J. Vis. Exp. (2014)This video demonstrates the assessment of viral replication capacity using a radiolabeled reverse transcriptase assay. Culture supernatants containing progeny virions are added to a reverse transcription mixture containing a radiolabeled nucleotide. The incorporated signal reflects viral output and replication...
03:28
Labeling Murine T Helper Lymphocytes using Radiolabeled Monoclonal Antibodies
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2025
In this video, we demonstrate the intracellular radiolabeling of T helper cells using radioactive isotope-conjugated monoclonal antibodies for non-invasive positron emission tomography imaging.
02:43
Thin-Layer Chromatography-Based Separation of (p)ppGpp Nucleotides Isolated from Stress-Induced Bacteria
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2025
Source: Fernández-Coll, L. and Cashel, M. Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography. J. Vis. Exp. (2019)This video presents a high-throughput protocol for measuring the levels and dynamics of the bacterial stress-response nucleotides ppGpp and pppGpp using thin-layer chromatography (TLC). The method provides a powerful, rapid, and cost-effective approach to quantifying (p)ppGpp in bacterial cultures...
10:01
Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster
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Cited by 7 •
2016
This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
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Cited by 15 •
2017
Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.
FAQs
When cells or biochemical systems use the tagged nucleotides as building blocks, the radioactive label becomes associated with newly produced DNA or RNA. Detecting the emitted radiation then provides a measurable signal linked to synthesis. Changes in signal can help researchers assess how much nucleic acid production occurs under particular experimental conditions.
The detected signal can provide information about the location, amount, or timing of a process. These dimensions allow researchers to examine not only whether nucleic acid synthesis or metabolism occurs, but also how strongly and when it changes. Such measurements support analysis of molecular interactions in cells and defined biochemical systems.
They create sensitive readouts for processes occurring in both pathogen and host cells. Researchers can follow pathogen replication while separately examining responses such as lymphocyte proliferation or other immune-cell activity. Comparing these signals helps reveal how infection alters nucleic acid-related processes and how host responses change during the interaction.
A researcher introduces the labeled building blocks into cells or a biochemical system and allows the relevant nucleic acid process to occur. The resulting radioactive emissions are then detected with an appropriate instrument, and the signal is interpreted as a measure of the process being studied. The workflow can be adapted to cellular or molecular assays.
The method is useful when investigators need to quantify pathogen replication, monitor lymphocyte proliferation, or analyze immune-cell responses. It can also support nucleic acid-based assays that examine molecular activity during infection. Because the signal is measurable, researchers can compare experimental conditions and evaluate changes associated with host-pathogen interactions.
Treatment effects can be assessed by measuring how radioactive signals change after an antimicrobial or immunomodulatory intervention. A difference in signal may indicate altered pathogen replication, nucleic acid synthesis, or immune-cell activity, depending on the assay design. This provides an experimental readout for comparing untreated and treated conditions in infection or immune-response studies.