Mouse Macrophages
Mouse Macrophages - Related Videos
03:14
Assessing the Survival of a Bacterial Pathogen in Mouse Peritoneal Macrophages
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2026
Source: Zhang, C. et al. Development and Assessment of Intracellular Infection Models for Staphylococcus aureus. J. Vis. Exp. (2025)This video demonstrates the quantification of intracellular survival of a methicillin-resistant bacterial pathogen in mouse peritoneal macrophages. It outlines the steps involved in infection, extracellular bacterial clearance, host cell lysis, and colony enumeration to assess bacterial survival within host macrophages.
09:25
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
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Cited by 68 •
2012
Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.
06:51
Activation of Mouse Bone Marrow-Derived Macrophages in Response to Antibodies
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2025
In this video, we demonstrate the activation of bone marrow-derived macrophages in response to bacterial lipopolysaccharide and intravenous immunoglobulin.
06:56
Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation
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2025
This video demonstrates an enzyme-free protocol to isolate macrophages from mouse dorsal root ganglion (DRG) using a mechanical dissociation technique. Upon homogenization of the freshly collected DRG tissue, antibodies against the cell-surface receptors on the macrophages are used to label the cells fluorescently, followed by performing flow cytometry to confirm the presence of macrophages in the sample.
12:47
Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
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Cited by 8 •
2014
In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.
FAQs
Pattern-recognition receptors connect microbial or tissue-derived signals with coordinated immune responses. Their engagement can promote phagocytosis, antimicrobial activity, antigen presentation, and the release of cytokines and chemokines. Studying this sequence helps immunologists determine how macrophages distinguish relevant danger signals and translate detection into pathogen clearance or inflammation during host defense.
Cytokines and chemokines provide measurable evidence that macrophages are coordinating inflammation rather than acting only as engulfing cells. Their secretion can indicate how strongly cells respond to microbial or tissue-derived cues and how those signals may influence surrounding immune activity. Comparing these outputs helps researchers examine inflammatory signaling during infection and tissue damage.
Environmental cues can shift macrophage behavior toward different combinations of antimicrobial activity, inflammatory signaling, and tissue-repair responses. This flexibility allows the same experimental system to reveal how local conditions affect disease progression or recovery. In infection studies, examining these changes helps connect the cellular response to broader interactions between microbes, host tissues, and immune regulation.
Primary macrophages and macrophage cell lines offer complementary experimental models. Primary cells support studies of macrophage behavior in a cellular system derived from mice, whereas cell lines provide an alternative model for examining pathogen clearance, inflammatory signaling, or microbe-host interactions. Choosing between them depends on which macrophage response or experimental comparison the study is designed to investigate.
Researchers can examine several connected outcomes, including pathogen clearance, phagocytosis, antimicrobial activity, antigen presentation, and secretion of cytokines or chemokines. Together, these observations show both what macrophages do to infectious material and how they communicate with the surrounding immune environment. The combination is useful for relating cellular behavior to host defense and inflammatory disease processes.
Mouse macrophage models are useful when a study needs to determine how an intervention affects host defense or inflammation. Researchers can examine whether treatment-related changes alter pathogen clearance, inflammatory signaling, or interactions between microbes and host tissues. These results provide cellular context for evaluating vaccines, antimicrobial treatments, and therapies intended to modify immune responses.