Spike Protein Detection
Spike Protein Detection - Related Videos
02:46
Visualizing Dose-Dependent Spike Protein Uptake with Quantum Dot Conjugates
0 Views •
2026
Source: Tran, B. N., et. al. High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells. J. Vis. Exp. (2022)This video demonstrates the use of quantum dot–conjugated spike protein (QD-Spike) to visualize dose-dependent viral entry in ACE2-GFP–expressing cells. Confocal imaging captures the internalization of QD-Spike via receptor-mediated endocytosis, modeling early coronavirus entry.
04:56
Antibody-Mediated Neutralization of Coronavirus Spike Protein-Expressing Pseudoviruses Using Patient Serum
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2026
Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the antibody-mediated neutralization of pseudoviruses carrying coronavirus spike proteins using patient serum, studied through high-throughput fluorescent imaging of infected epithelial cell monolayers.
02:19
Triggering a Closed-Loop Stimulation by Neuron Spiking Activity
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2025
This video demonstrates the operation of closed-loop stimulation in a rat with a surgically implanted device to record neural activity. Neuronal signals are first recorded and analyzed. Template waveforms are then uploaded, and parameters are defined to trigger closed-loop stimulation.
08:29
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
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Cited by 23 •
2014
An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.
09:00
Detection of Protein Ubiquitination
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Cited by 96 •
2009
Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.
FAQs
Capture and detection antibodies translate molecular recognition into a measurable result. The capture antibody binds a spike-protein epitope, while the detection antibody contributes to signal generation after recognizing the target. This paired recognition links the presence of a specific viral surface molecule to an immunoassay or rapid-test readout.
Epitope specificity determines whether the antibodies can recognize the intended region of spike protein. Because these regions also serve as targets for immune recognition, antibody binding has two connected implications: it enables analytical detection and provides a molecular basis for studying immune interactions with the viral surface. This principle is central to developing and validating detection assays.
Signal comparisons are useful because they place measurements from different samples into an interpretable pattern. Researchers can use relative spike-protein signals to characterize infection, monitor immune protection, and assess vaccine-induced immunity. The comparison does not stand alone; its value comes from applying the same detection approach across samples so the resulting measurements can be evaluated consistently.
Spike protein detection and antibody-response evaluation address related but distinct questions. Detecting spike protein supports identification of viral material, whereas evaluating antibody responses examines immune recognition associated with infection or vaccination. Keeping these purposes separate helps researchers interpret a signal correctly and select measurements suited to pathogen detection, immune assessment, or both.
A basic antibody-based workflow begins by presenting a sample to a capture antibody that can bind a spike-protein epitope. A detection antibody then recognizes the target and supports signal production. Measuring that signal provides the assay result, while the chosen antibody specificities determine whether the format addresses the intended spike-protein target.
Identified formats include immunoassays and rapid antigen tests. The appropriate format depends on the research or diagnostic question, including pathogen detection, comparison of samples, or assessment of immune responses. Using a defined format also supports assay development and validation by providing a consistent way to evaluate spike-protein signals.
In infection research, measurements can connect detection with immunological interpretation. Spike-protein signals may help characterize infection, while related analyses support evaluation of antibody responses and vaccine-induced immunity. Together, these uses allow investigators to examine both evidence relevant to the pathogen and the immune protection associated with exposure or vaccination.
Beyond identifying infection, the measurements can inform development of diagnostic assays and therapeutic strategies. Researchers may compare signals during assay validation, use the results to characterize immune protection, and examine how spike protein serves as a target for immune recognition. This makes the technique relevant to both test development and infection-focused immunology.