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August 19, 2009
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Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.
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Protein UbiquitinationPosttranslational ModificationUbiquitin-activating Enzyme E1Ubiquitin-conjugating Enzyme E2Ubiquitin Ligase E3Deubiquitylating EnzymesProtein StabilityCellular LocalizationBiological ActivityGene MutationsHuman DiseasesCancerNeurodegenerationMetabolic DisordersPathogenesisDetection ProtocolsCultured Cells In VivoTest Tubes In VitroSumolyationNeddylation
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Choo, Y. S., Zhang, Z. Detection of Protein Ubiquitination. J. Vis. Exp. (30), e1293, doi:10.3791/1293 (2009).
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