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Biology

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Detection of Protein Ubiquitination
 

Detection of Protein Ubiquitination

Article DOI: 10.3791/1293
August 19th, 2009

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Summary August 19th, 2009

Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.

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