Spleen Immune Cells
Spleen Immune Cells - Related Videos
08:04
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
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Cited by 10 •
2019
This protocol details the surgical steps of a mouse model of vascularized heterotopic spleen transplantation, a technically challenging model that can serve as a powerful tool in studying the fate and longevity of spleen cells, the mechanisms of distinct spleen cell populations in disease progression, and transplant immunity.
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Cited by 22 •
2008
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
04:47
A Mouse Model of Vascularized Heterotopic Spleen Transplantation
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2025
The video demonstrates a surgical method for generating a mouse model of vascularized heterotopic spleen transplantation. The abdominal cavity of an anesthetized donor mouse is surgically opened to remove the spleen. The inferior vena cava and infrarenal aorta are identified, their lumbar branches are ligated, and openings are made for the heterotopic transplantation of a spleen from a congenic donor mouse. Upon completing vascular anastomosis and restoring blood flow to the implanted spleen,...
03:06
Developing a Humanized Mouse Model with Human Liver Cells and Immune Cells
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2025
This video demonstrates a method for generating a dual humanized mouse model with a human immune system and liver cells. It involves transplanting human liver cells and hematopoietic stem and progenitor cells into a transgenic immunodeficient mouse via intrasplenic injection, leading to engraftment of liver cells and development of human immune cells.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
FAQs
Coordination begins when macrophages and dendritic cells capture microbes or microbial antigens from circulating blood. Dendritic cells and other antigen-presenting cells then display antigenic information to lymphocytes, linking early detection with targeted immunity. Activated B cells can produce antibodies, while T cells and natural killer cells contribute cellular responses, allowing several immune mechanisms to operate together.
Different leukocyte populations occupy distinct splenic regions, positioning them for complementary tasks. Cells involved in microbial capture and antigen presentation can encounter material arriving through the blood, while lymphocytes respond in specialized areas that support activation and expansion. This organization helps the spleen combine blood surveillance, immune communication, and the development of targeted responses.
Macrophages and dendritic cells emphasize microbial capture and antigen presentation, whereas B cells support antibody production. T cells provide targeted cellular immune activity, and natural killer cells contribute cytotoxic responses. These roles are not interchangeable: antigen-presenting cells initiate immune recognition, while lymphocyte and natural killer cell activities help produce specific or cell-directed effects against infection.
Because blood passes through the spleen, immune cells can monitor material circulating throughout the body rather than sampling only a local tissue site. This exposure enables macrophages and dendritic cells to encounter blood-borne microbes and debris. The resulting capture and presentation processes connect filtration of the blood with systemic immune activation during infection.
Studies of these cells can examine how systemic infections are detected, how inflammation is coordinated, and how antibody or cellular responses develop. Researchers can also assess memory responses after immune activation and investigate how splenic organization supports both blood filtration and immunity. These outcomes make the spleen relevant to infection biology, vaccine research, and immune disorders.
The spleen provides a setting in which circulating antigens can be captured and presented to lymphocytes. B-cell responses can then be examined alongside T-cell activity and the development of targeted or memory responses. This makes splenic immune cells useful for studying how vaccination-related immune recognition progresses from antigen exposure toward longer-lasting protection.