Viral Titration Assay
Viral Titration Assay - Related Videos
12:00
Titration of Human Coronaviruses Using an Immunoperoxidase Assay
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Cited by 14 •
2008
In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.
04:32
Plaque Titration Assay for Quantification of Infectious Respiratory Syncytial Virus
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2026
Source: Bouillier, C., et al. Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses. J. Vis. Exp. (2019)The video demonstrates a plaque titration assay used to quantify infectious recombinant respiratory syncytial virus (RSV) in epithelial cell monolayers. It presents the infection of host cells with serially diluted viral suspensions, the restriction of viral spread by a cellulose-based semi-solid overlay, and the formation of localized plaques from cytopathic...
04:06
Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays
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Cited by 9 •
2022
This protocol shows an accurate and objective approach to visualize viral titrations using crystal violet, by comparing it with optical microscopy and immunocytochemical staining.
13:45
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
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Cited by 84 •
2011
The Virochip is a pan-viral microarray designed to simultaneously detect all known viruses as well as novel viruses on the basis of conserved sequence homology. Here we demonstrate how to run a Virochip assay to analyze clinical samples for the presence of both known and unknown viruses.
10:17
Introduction to Titration
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2023
Source: Laboratory of Dr. Yee Nee Tan — Agency for Science, Technology, and Research Titration is a common technique used to quantitatively determine the unknown concentration of an identified analyte.1-4 It is also called volumetric analysis, as the measurement of volumes is critical in titration. There are many types of titrations based on the types of reactions they exploit. The most common types are acid-base titrations and redox titrations.5-11 In a typical titration process, a standard...
FAQs
Plaque-forming units and 50% tissue culture infectious dose describe infectious virus using different measurement conventions. Plaque-based results rely on counting visible plaques produced in susceptible cells, whereas the 50% tissue culture infectious dose reflects the amount associated with infection in half of the tissue-culture assessment. The selected format should match the assay’s detectable outcome and experimental comparison.
Susceptible cells provide the biological system in which infectious virus can produce a detectable result. Depending on the assay design, infection may appear as discrete plaques, visible cytopathic effects, or another measurable signal. Because the readout depends on infection in these cells, the cell system connects the virus preparation to an experimentally observable measure of infectivity.
Serial dilution creates a range of virus concentrations that can be evaluated across the same susceptible-cell system. This range helps researchers relate the amount of virus added to the resulting infection signal rather than relying on a single concentrated sample. The resulting measurements support comparisons among virus preparations, experimental conditions, or samples with different levels of infectivity.
A viral titration assay may quantify infection through plaque formation, cytopathic effects, or another detectable signal supported by the experimental system. Plaques provide a countable manifestation of localized infection, while cytopathic effects record virus-associated changes in the cells. Choosing a suitable readout allows the assay to express infectious virus in a form appropriate for the study.
A typical workflow begins with preparing serial dilutions of the virus sample, followed by exposing susceptible cells to the dilution series. The researcher then identifies the infection signal, such as plaques or cytopathic effects, and reports the result using an appropriate infectious-virus measure. This workflow converts a virus preparation into a quantitative value for downstream experiments.
In vaccine research, titration helps characterize viral infectivity and evaluate immune responses. In antiviral studies, it supports comparisons of viral replication across samples or experimental conditions. The assay can also help define the dose used in an experiment, making it possible to relate treatment or immune effects to a measured amount of infectious virus rather than to an unquantified preparation.