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Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
This content is Free Access.
Chapters
- 00:05Title
- 01:00Introduction
- 02:23Nucleic Acid Extraction
- 03:45Reverse Transcription and 2nd-strand cDNA Synthesis (“Round A”)
- 05:36PCR Amplification of Randomly Primed cDNA
- 06:40Cy3 Fluorescent Dye Incorporation
- 08:02Virochip Microarray Hybridization, Scanning and Analysis
- 10:34Virus Detection Using Virochip Microarray
- 12:15Conclusion
The Virochip is a pan-viral microarray designed to simultaneously detect all known viruses as well as novel viruses on the basis of conserved sequence homology. Here we demonstrate how to run a Virochip assay to analyze clinical samples for the presence of both known and unknown viruses.
Tags
Pan-Viral Microarray AssayVirochipClinical SamplesViral PathogensViral DiagnosisSequence DiversityNovel Viral PathogensSARS CoronavirusH1N1 Influenza VirusTraditional MethodsPan-viral Microarray PlatformConserved Sequence HomologyRespiratory InfectionsUnexplained Critical IllnessConventional Clinical TestingNovel VirusesRhinovirus CladeXMRVAvian BornavirusCardiovirusAgilent Microarray PlatformGenBank
