4t1 Tumor Model
4t1 Tumor Model - Related Videos
03:46
Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model
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2025
Source: Marina Arantes Radicchi1, Victor Carlos Mello1, Victória Paz Machado1, Márcia Cristina Oliveira da Rocha1, Jaqueline Vaz de Oliveira1, Luana Cristina Camargo1,2, Luís Alexandre Muehlmann1, Ricardo Bentes Azevedo1, Sônia Nair Bao1, João Paulo Figueiró Longo11Institute of Biological Sciences, University of Brasília, 2Department of Basic Psychological Processes, Institute of Psychology, University of BrasíliaThis video demonstrates the procedure for tracking tumor progression in vivo by...
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
03:17
Preparing Organoids from Tumors: A 3D In Vitro Tumor Model
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2023
Organoids derived from tumors are a type of 3D cell culture model that can maintain the pathological features and organ-specific cell lineage of tumors. In the example protocol, we will see scientists generate organoids from a prostate tumor from a genetically engineered mouse model.
06:23
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
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Cited by 8 •
2017
This protocol describes the generation of murine orthotopic bladder tumors in female C57BL/6J mice and the monitoring of tumor growth.
FAQs
Its syngeneic design allows 4T1 mammary carcinoma cells to grow in a genetically compatible, immunocompetent BALB/c mouse. This preserves interactions between the tumor and the host immune system rather than isolating cancer cells from immune influences. Consequently, treatment responses can be interpreted in the context of antitumor immunity as well as direct effects on tumor cells.
Spontaneous dissemination links growth of the mammary primary tumor with metastatic progression to distant tissues. This enables investigators to examine invasion and metastasis as developments arising during tumor progression, rather than studying only an isolated primary mass. The model therefore supports questions about whether a treatment affects local tumor growth, dissemination, or both.
The model retains interactions among cancer cells, stromal tissue, and immune cells within the tumor environment. These relationships provide a setting for examining how the surrounding tissue and host defenses relate to tumor growth, invasion, metastatic progression, and antitumor immunity. Such context is especially relevant when evaluating therapies whose effects may depend on the immune system.
Because the model produces a mammary primary tumor followed by dissemination to distant tissues, researchers can consider these outcomes as related but distinct experimental readouts. A treatment may be evaluated for its influence on primary tumor growth, metastatic progression, or both. This separation helps clarify whether an intervention primarily controls local disease or broader progression.
The usual setup begins with implantation of 4T1 mammary carcinoma cells into the mammary fat pad of BALB/c mice. The implanted cells then form a primary tumor and can disseminate during progression. This arrangement provides a consistent biological context for studying tumor development and testing interventions in an immunocompetent host.
The 4T1 Tumor Model supports evaluation of chemotherapy, immunotherapy, and combination treatments. Using the same tumor system, investigators can examine responses to approaches that act directly against cancer cells, engage antitumor immunity, or combine both strategies. The model also provides context for studying why tumors may show treatment resistance during progression.
Studies can address tumor growth, invasion, metastatic progression, antitumor immunity, and treatment resistance. Because the model includes a primary tumor, distant dissemination, stromal tissue, and immune interactions, it supports broader assessment than primary tumor size alone. These outcomes help investigators connect therapeutic response with the biological processes underlying aggressive breast cancer.