Histological Parenchymal Biopsies
Histological Parenchymal Biopsies - Related Videos
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
03:41
Preparing Cerebral Endothelial Tubes from Mouse Parenchymal Arterioles
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2025
This video demonstrates a method for isolating the endothelial tubes from mouse cerebral parenchyma. Through a combination of enzymatic digestion and mechanical dissociation, the process yields an intact tube with endothelial cells.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
08:05
Detection and Isolation of Cancer in Prostate Biopsies Using Stimulated Raman Histology and Artificial Intelligence
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Cited by 1 •
2025
Here, we present a standardized protocol for detecting prostate cancer using stimulated Raman histology (SRH) on biopsy samples due to advantages over traditional histopathology, focusing on sample preparation, imaging, and artificial intelligence to improve the cancer-to-tissue ratio. Additionally, it supports biobanking for transcriptomics, organoid or xenograft models, and identification of intraoperative surgical margins.
09:01
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
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Cited by 7 •
2017
We standardized an abdominal adipose tissue biopsy using a non-diathermy method performed under local anesthesia. Three cases of excessive post-operation bleeding out of 115 operations (2.61%) occurred.We conclude that an abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men.
FAQs
Tissue architecture shows how cells and structural elements are arranged within the organ, whereas cell morphology focuses on the appearance of individual cells. Examining both levels helps identify structural and cellular abnormalities rather than relying on a single feature. Their combined assessment can support disease classification and provide evidence about the nature and extent of tissue change.
Evaluation commonly considers tissue architecture, cell morphology, inflammation, fibrosis, and other disease-associated features. These findings can be assessed together to recognize patterns of abnormality and estimate disease severity. Because no single feature necessarily provides the full picture, combining cellular and structural observations strengthens the tissue-based interpretation and supports decisions about diagnosis or classification.
Different disease processes may produce different combinations of architectural disruption, cellular abnormalities, inflammation, or fibrosis. Microscopic assessment makes these changes directly visible in preserved tissue sections, allowing the observed pattern to be compared with disease-associated features. This tissue-based evidence can help separate conditions that may otherwise require classification according to their microscopic effects on an organ.
After collection, the specimen is preserved, processed, cut into thin sections, and stained before microscopic evaluation. Preservation maintains the sample for subsequent handling, while thin sections allow the tissue to be examined in detail. Staining enhances relevant structural or cellular features, enabling assessment of architecture, morphology, inflammation, fibrosis, and other abnormalities.
The approach is relevant to biopsies from organs including the liver, kidney, lung, and gastrointestinal tract. In each setting, microscopic findings can reveal abnormalities within the organ’s functional tissue and help characterize the associated disease process. The resulting evidence may support diagnosis, classification, severity assessment, and treatment-related decisions.
Clinical interpretation can use biopsy findings to estimate disease severity and guide treatment decisions, while research can use the same tissue-based evidence to investigate disease mechanisms and therapeutic response. Comparing structural or cellular abnormalities across samples provides information about how disease is expressed in tissue and how that expression may change following treatment.