Monocytic Lineage
Monocytic Lineage - Related Videos
07:58
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
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Cited by 12 •
2012
We describe use of ImageStream technology (www.amnis.com), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging, to quantify cellular mechanisms of primary immune cells from well-defined patient cohorts. Our studies provide a blueprint for translational investigations to quantify lineage specific cellular responses in small samples from subject cohorts.
04:19
Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method
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Cited by 2 •
2022
Here we present a protocol for the isolation of BMMs from SD rats, called the secondary adherence method.
08:31
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Cited by 19 •
2016
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
04:27
Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection
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2025
In this video, we demonstrate the isolation of monocytes from whole human blood by immunomagnetic negative separation. The isolated monocytes can be used for further downstream analysis.
03:05
In Vitro Culture and Differentiation of Primary Monocytes into Macrophages
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2025
This video demonstrates the priming of primary monocytes into macrophages. Primary human monocytes can be isolated and differentiated to an M1-like pro-inflammatory phenotype using the granulocyte-macrophage colony-stimulating factor (GM-CSF) or to an M2-like anti-inflammatory phenotype using the macrophage colony-stimulating factor (M-CSF).
FAQs
Development proceeds through successive bone-marrow stages, including the monoblast and promonocyte, before cells are released as circulating monocytes. This progression reflects progressively specialized myeloid development rather than an immediate change into a mature phagocytic cell. Following circulation, the cells can respond to tissue signals and continue differentiating according to local needs.
Cytokines and growth factors regulate several linked features of monocytic development, including cell differentiation, survival, and activation. Their influence helps control how progenitor cells progress through marrow stages and how resulting monocytes respond after release. This regulatory network is important because altered control can affect both immune function and the development of myeloid disorders.
After entering tissues, monocytes can differentiate into macrophages or certain dendritic cells, allowing the lineage to support more than one specialized immune role. Macrophage differentiation supports engulfment of pathogens and damaged material, while dendritic-cell development contributes to coordination of immune responses. This tissue-dependent specialization connects blood-cell production with local maintenance and inflammation.
Blood counts provide clinical information about circulating monocytes and can help clinicians evaluate whether monocytic production or distribution may be relevant to disease. These results are interpreted in the context of suspected infection, inflammatory disorders, or abnormal myeloid development. The lineage therefore links routine hematologic assessment with broader diagnostic investigation.
The lineage becomes particularly relevant when clinicians investigate infections or inflammatory disorders because monocytes and their tissue-derived descendants participate in innate immune defense and inflammatory coordination. Their ability to engulf pathogens, remove damaged material, and support immune signaling makes monocytic findings useful as part of the clinical assessment of these conditions.
Monocytic leukemias and related diseases can involve abnormal myeloid development within this lineage. Understanding the normal sequence from hematopoietic progenitors through marrow stages and circulating monocytes provides a framework for recognizing when development is disrupted. In medicine, this context supports interpretation of blood findings and investigation of disorders involving monocytic cells.