Pancreatic Steatosis

Pancreatic steatosis is the abnormal accumulation of fat within the pancreas, a condition increasingly recognized in metabolic and clinical medicine. It develops when excess lipid deposition, often associated with obesity, insulin resistance, or metabolic syndrome, alters pancreatic tissue and may impair endocrine and exocrine function. Clinicians assess pancreatic fat using imaging techniques such as ultrasound, computed tomography, and magnetic resonance imaging, although standardized diagnostic criteria remain under development. Studying pancreatic steatosis may clarify its links to type 2 diabetes, pancreatitis, and pancreatic disease, supporting earlier risk assessment and more targeted approaches to prevention and treatment.

Pancreatic Steatosis - Related Videos

Research

JoVE Journal - Medicine

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

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Cited by 28 •

2017

Here, optimized methods to generate in vivo and in vitro models of hepatic steatosis and to analyze the steatotic phenotypes and related physiological parameters are described.

Oil Red O Staining: A Technique for Staining Neutral Lipids in Hepatocytes for Detecting Hepatic Steatosis In Vitro

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2025

In this video, we demonstrate in vitro staining of neutral lipids in hepatocytes to detect hepatic steatosis or excessive fat accumulation in liver. Excessive fat is accumulated in the cells in form of lipid droplets. Oil Red O is a lipid soluble dye that binds to intracellular neutral lipids in these lipid droplets. When observed under a light microscope, these intracellular lipid droplets appear intensely red in color.

Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion

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2025

This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

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Cited by 20 •

2009

Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part I: digestion and collection of pancreatic tissue) using a modified automated method.

Treatment of Middle-segment Pancreatic Benign Tumor Using Laparoscopic Central Pancreatectomy with End-to-end Pancreatic Duct Reconstruction

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2026

This article presents laparoscopic central pancreatectomy with pancreatic duct end-to-end anastomosis as an important surgical approach for middle-segment pancreatic benign and low-grade malignant tumors.

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