Pediatric Allergen Sensitization
Pediatric Allergen Sensitization - Related Videos
05:37
Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry
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2025
In this video, we demonstrate the test to evaluate basophil activation in the presence of a test allergen using flow cytometry.
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
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2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
10:42
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Cited by 8 •
2016
Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
08:05
Murine Model of Allergen Induced Asthma
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Cited by 51 •
2012
Experimental mouse models of allergic asthma offer new possibilities for studying disease pathogenesis and developing new therapeutics. These models are well suited to measuring factors governing the allergic immune response, airway inflammation, and pulmonary pathophysiology.
FAQs
Antigen-presenting cells initiate the adaptive immune response by activating allergen-specific T cells, which then support activation of allergen-specific B cells. This coordinated cellular response leads to production of immunoglobulin E, or IgE, directed against the otherwise harmless substance. The sequence explains how immune recognition becomes established before a later exposure is assessed clinically.
Allergen-specific IgE binds to mast cells and basophils after it is produced. These cells are therefore prepared to release inflammatory mediators when the child encounters the relevant allergen again. Their involvement connects the earlier adaptive immune response with the potential inflammatory response during subsequent exposure, making IgE an important marker of the sensitization pathway.
Sensitization demonstrates immune reactivity to an allergen, but it does not by itself establish confirmed allergy. Clinical interpretation must consider the child’s history alongside testing results, because identifying allergen-specific IgE or a response on skin-prick testing indicates immune recognition rather than automatically proving that exposure produces allergic disease. This distinction supports more accurate risk assessment.
Evaluation may focus on food proteins, pollen, dust mites, or animal dander because these are examples of otherwise harmless substances that can induce allergen-specific immune responses. Considering the relevant exposure helps clinicians connect testing with the child’s medical history and determine which findings may be useful for risk assessment, monitoring, or management decisions.
Assessment combines clinical history with tests such as skin-prick testing or measurements of allergen-specific IgE. The history identifies relevant exposures, while testing evaluates immune reactivity to selected allergens. Used together, these approaches help distinguish sensitization from confirmed allergy and provide information that can guide subsequent avoidance, monitoring, and treatment strategies.
Sensitization results can inform risk assessment and help clinicians decide which allergens require attention in a child’s care. Depending on the overall clinical picture, the findings may support strategies involving avoidance, monitoring, or treatment. Their value lies in integrating laboratory or skin responses with clinical history rather than using a test result as the sole basis for conclusions.