Recipient Derived Vessels
Recipient Derived Vessels - Related Videos
10:52
Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
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Cited by 22 •
2014
Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.
12:52
Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA First discovered by Lederberg and Tatum in 1946, conjugation is a form of horizontal gene transfer between bacteria that relies on direct physical contact between two bacterial cells (1). Unlike other forms of gene transfer, such as transformation or transduction, conjugation is a naturally occurring process in which...
03:15
A Peptide Array-Based Detection of Anti-donor HLA Alloantibodies in Organ Recipients
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2025
This video demonstrates an assay to detect anti-donor HLA alloantibodies in organ recipients. A peptide array is synthesized to represent potential epitopes for antibody-mediated organ rejection. The array is incubated with the recipient's serum, containing alloantibodies that bind to their target epitopes. Antibody binding is detected using chemiluminescence to identify donor-specific HLA epitopes that incite alloantibody reactivity.
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
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Cited by 80 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
11:21
Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA Transduction is a form of genetic exchange between bacteria that utilizes bacteriophages, or phages, a class of virus that infects exclusively prokaryotic organisms. This form of DNA transfer, from one bacterium to another by way of a phage, was discovered in 1951 by Norton Zinder and Joshua Ledererg, who termed the...
FAQs
Vascular anastomosis creates a connection between a patient’s vessel and the vessels supplying or draining a graft or reconstructed tissue. The connection must support both incoming and outgoing blood flow, rather than simply placing tissues in contact. When the linkage functions adequately, circulation reaches the transferred region and helps sustain graft viability, wound healing, and recovery.
Caliber and location influence whether a recipient vessel can be technically connected to the graft or reconstructed tissue. A suitable vessel must be accessible in the operative region and sufficiently compatible in size and position for the planned connection. These factors determine technical feasibility and help surgeons anticipate whether reliable perfusion can be established.
Blood-flow quality affects whether the connected tissue receives adequate circulation after reconstruction or transplantation. Poor or unsuitable flow can compromise perfusion, whereas an appropriate recipient vessel supports delivery of blood to the graft and drainage away from it. Evaluating flow quality therefore contributes to vessel selection, surgical planning, and the likelihood of graft survival.
Tissue compatibility is one of several considerations in deciding whether a patient’s vessel is appropriate for a graft or reconstructed region. Along with caliber, location, and blood-flow quality, compatibility affects the practicality of establishing a dependable vascular connection. Considering these features together supports better perfusion and may improve wound healing and functional recovery.
Preparation begins with selecting recipient vessels according to their location, caliber, blood-flow quality, and tissue compatibility. Surgeons then prepare those vessels for vascular anastomosis with the graft or reconstructed tissue, creating routes for inflow and outflow. This process links recipient circulation to the operative construct and is central to maintaining postoperative perfusion.
They are particularly important in transplantation and reconstructive surgery, where a graft or repaired region requires a circulation established through the patient’s own vessels. Their selection also informs work on vascularized tissues and regenerative therapies. In each setting, the objective is to support perfusion sufficiently for graft survival, wound healing, and restoration of function.