Thread Embedding

Thread embedding is a minimally invasive medical technique in which absorbable threads are inserted into selected subcutaneous sites or acupuncture points to provide sustained local stimulation. As the threads gradually degrade, their placement may produce mechanical stimulation and a localized tissue response, while the procedure can be tailored by selecting different materials, insertion depths, and anatomical locations. In clinical and research settings, thread embedding has been investigated within integrative medicine and aesthetic practice for symptom management, tissue support, and longer-lasting effects than brief needle-based stimulation. Careful patient selection, sterile technique, and monitoring for pain, infection, inflammation, or other complications are essential.

Thread Embedding - Related Videos

Research

JoVE Journal - Neuroscience

Operation Procedure and Precautions of Thread-Embedding Acupuncture Therapy in Alzheimer

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2024

This study presents a protocol for thread-embedding acupuncture therapy in Alzheimer's disease-like rats.

Inducing Ischemic Stroke via Middle Cerebral Artery Occlusion Using a Thread Bolt

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2025

This video demonstrates the process of inducing ischemic stroke in a rat model by occluding the middle cerebral artery (MCA) using a thread bolt. The procedure involves ligating the carotid arteries, inserting a thread bolt into the external carotid artery, and blocking blood flow from the MCA to the brain, leading to neuronal death and mimicking an ischemic stroke.

Embedding Adult Drosophila Heads in Paraffin: Preparing Tissue for Histology

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2023

This video describes how to embed Drosophila heads in paraffin using the collar method, a technique that prepares fly heads for serial sectioning. Paraffin sections are used for histological examinations of the brain's anatomy and for the assessment of neurodegeneration. The featured protocol demonstrates how multiple flies can be processed in one preparation, thereby increasing time-efficiency and minimizing experimental artifacts.

Quantification of Tear Volume in an Autoimmune Dry Eye Rat Model using the Phenol Red Thread

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2025

This video demonstrates the phenol red thread test to determine tear volume in an autoimmune dry eye rat model. The pH-sensitive acidic phenol red thread changes color when it comes in contact with the alkaline tears. The shorter length of color change in the phenol red thread corresponds to reduced tear volume.

Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery

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2025

This protocol describes a unique clinical protocol for collecting human tear fluid samples using phenol red threads and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based workflow to discover the tear lipidomic profile. The simple methyl tert-butyl ether (MTBE)/methanol biphasic separation method enables rapid tear lipid extraction with high recovery for tear biomarker discovery.

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