Tissue Microarray Construction
Tissue Microarray Construction - Related Videos
12:03
Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
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Cited by 5 •
2022
This protocol outlines the tape method on how to manually construct a tissue microarray using FFPE donor blocks of differing depths.
13:43
Fabrication of Myogenic Engineered Tissue Constructs
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Cited by 11 •
2009
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
09:08
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
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Cited by 202 •
2012
Tissue microarrays allows for an efficient method to gain concurrent information from a multitude of tissues. Representative parts of tissues are assembled into a single paraffin block. Sections from the block are used for immunohistochemistry and analysis of protein expression patterns. Digital scanning generates corresponding images for distribution of data.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
08:34
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Cited by 59 •
2016
We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.
FAQs
The critical design principle is representativeness: each core must capture a defined region of the donor tissue that is relevant to the biological question. Placing these cores in a mapped grid lets investigators identify the source of each specimen after sectioning and compare markers across many donors. This organization supports efficient, consistent analysis rather than isolated specimen-by-specimen evaluation.
A common recipient paraffin block places cores from multiple donor specimens under shared sectioning and staining conditions. That arrangement improves assay standardization because marker measurements can be compared across samples processed together. It also makes efficient use of limited tissue, which is especially important when specimens are valuable or available only in small amounts.
Precise grid mapping preserves the link between a stained location and its original donor specimen. This information is essential when investigators compare biological markers across cases, because a signal can be interpreted in relation to the correct tissue source. Mapping therefore converts a densely packed block into an organized dataset suitable for biomarker validation and disease-focused comparisons.
Construction proceeds from donor-block selection to identification of defined regions in formalin-fixed, paraffin-embedded tissue. A tissue arrayer removes and transfers cores into predetermined positions within a recipient paraffin block, while the grid layout is recorded. The completed block is then sectioned, and the resulting sections can undergo parallel staining for comparative analysis.
Sections produced from a completed array can support immunohistochemistry, in situ hybridization, and other molecular assays. Because many donor-derived cores appear on the same section, investigators can examine the selected marker across numerous specimens under consistent experimental conditions. This makes the technique useful for comparing biological markers during pathology and biomarker-focused studies.
Medical researchers use arrays when they need to evaluate biomarkers across many tissue specimens efficiently while conserving valuable material. Applications include biomarker validation, diagnostic research, and investigations of disease progression or treatment response. The shared format accelerates these comparisons and supports more standardized assessment than examining each donor specimen in isolation.