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Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
JoVE Journal
Biology
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JoVE Journal Biology
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
DOI:

09:08 min

May 31, 2012

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Chapters

  • 00:05Title
  • 01:38Manual Tissue Microarray Production
  • 05:59How to Search for a Protein of Interest in the Human Protein Atlas
  • 07:06Representative Tissue Microarray Results
  • 08:35Conclusion

Summary

Automatic Translation

Tissue microarrays allows for an efficient method to gain concurrent information from a multitude of tissues. Representative parts of tissues are assembled into a single paraffin block. Sections from the block are used for immunohistochemistry and analysis of protein expression patterns. Digital scanning generates corresponding images for distribution of data.

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