Drg Tissue Analysis
Drg Tissue Analysis - Related Videos
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
04:25
Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells
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2025
This video demonstrates a procedure for isolating dorsal root ganglion (DRG) neurons from rat embryos and co-culturing them with Schwann cell precursors to generate mature Schwann cells. The isolated DRGs are processed to isolate neurons and glial cells. The isolated cells are cultured to purify the neurons, which are then co-cultured with Schwann cell-like cells (SCLCs) in a co-culture medium. Interaction with the DRG neurons leads to the maturation of SCLCs into Schwann cells.
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
04:38
Analysis of Immune Cells in Mouse Nervous Tissues Using Flow Cytometry
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2025
Source: Hidmark, A. S., et al. Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry. J. Vis. Exp. (2017). This video demonstrates the use of flow cytometry to analyze immune cells in mouse sciatic nerves and dorsal root ganglia (DRGs). The process involves labeling immune cells with fluorophore- and biotin-tagged antibodies, stabilizing and permeabilizing the cells, and using fluorescent markers to detect cell-surface and nuclear...
FAQs
The analysis combines tissue organization with marker expression and microscopic appearance. Histological stains reveal overall structure, while immunolabeling identifies particular cellular or molecular markers within the dorsal root ganglion. Examining these signals alongside cell morphology helps distinguish sensory neuron populations from satellite glial cells and reveals whether injury or disease is associated with altered cellular composition or tissue structure.
These methods contribute different layers of evidence. Histological stains show general tissue architecture, immunolabeling localizes selected markers, and microscopy records the resulting cellular and structural patterns. Used together, they allow investigators to examine molecular composition, inflammatory signals, sensory neuron populations, and pathology rather than relying on a single visual or molecular measurement.
Satellite glial cells and inflammatory signals provide indicators of the tissue environment surrounding sensory neurons. Their distribution or marker expression can be examined alongside neuronal features to identify changes associated with nerve damage, neuroinflammation, or disease. This parallel assessment helps connect local tissue alterations with mechanisms that may influence peripheral sensory pathways and pain-related outcomes.
A typical workflow begins by preserving the dorsal root ganglion tissue and preparing sections for examination. Researchers then apply suitable histological stains or immunolabeling procedures, image the sections with microscopy, and evaluate cellular organization, marker expression, morphology, or pathology. The selected measurements depend on whether the study focuses on sensory populations, inflammation, structural change, or injury responses.
Quantification may focus on cell morphology, the expression of selected markers, or visible tissue pathology. These measurements convert microscopic observations into data that can be compared across experimental conditions or disease-related changes. When interpreted with the tissue’s cellular organization, the results can help relate molecular and structural alterations to functional outcomes in peripheral sensory pathways.
It is especially useful when investigators need tissue-level evidence about sensory pathways after nerve damage or during disease. Studies of neuropathic pain can examine neuronal and glial changes, whereas neuroinflammation research can assess inflammatory signals and related pathology. The approach also supports evaluation of potential therapeutic interventions by showing whether tissue features or molecular markers change.
DRG findings can connect changes in sensory neuron populations, satellite glial cells, inflammatory signals, or tissue morphology with pain-related processes. This provides a cellular and molecular context for interpreting altered peripheral sensory function. In intervention studies, shifts in marker expression or pathology can help determine whether a treatment is associated with changes in the examined tissue.