Endothelial Cell Characterization
Endothelial Cell Characterization - Related Videos
09:58
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Cited by 2 •
2017
This manuscript describes the efficient, non-viral delivery of miR to endothelial cells by a PEI/MNP vector and their magnetization. Thus, in addition to genetic modification, this approach allows for magnetic cell guidance and MRI detectability. The technique can be used to improve the characteristics of therapeutic cell products.
12:50
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
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Cited by 1 •
2017
Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.
13:46
Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood
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Cited by 46 •
2012
Endothelial colony forming cells (ECFCs) are circulating endothelial cells with robust clonal proliferative potential that display intrinsic in vivo vessel forming ability. Phenotypic and functional characterization of outgrowth endothelial cells derived from CB are important to identify and isolate bona fide ECFCs for potential clinical application in repairing damaged tissues.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
2010
We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
10:15
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
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Cited by 20 •
2015
We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.
FAQs
No single measurement fully establishes an endothelial phenotype. Microscopy shows cellular appearance, immunodetection identifies proteins associated with endothelial identity, and permeability or barrier assays test whether cells restrict solute passage. Agreement among these measurements provides a stronger profile and helps distinguish endothelial characteristics from isolated marker expression or morphology alone.
These markers provide complementary molecular evidence. CD31/PECAM-1 and von Willebrand factor support identification of endothelial cells, while VE-cadherin is associated with endothelial cell junctions. Examining the pattern and presence of multiple markers is more informative than relying on one signal, particularly when comparing endothelial phenotypes or assessing changes in a model.
Cell junctions help determine how readily substances cross an endothelial layer. Barrier-function assays assess permeability or solute passage, so altered junctional organization can produce a measurable change even when cells remain present. In neuroscience models, these results are especially relevant for evaluating properties associated with the blood-brain barrier and communication between blood and neural tissue.
Morphology provides visual evidence of cellular organization, whereas barrier testing examines performance under transport conditions. A characterization profile that combines both can reveal differences between endothelial phenotypes and identify functional changes that microscopy alone may not show. This comparison is useful when studying vascular development, injury, inflammation, or disease-related dysfunction.
A typical workflow begins with microscopy to document morphology, followed by immunodetection of endothelial markers such as CD31/PECAM-1, von Willebrand factor, and VE-cadherin. Functional testing then evaluates permeability, barrier behavior, or solute passage. Considering these results together produces a multidimensional assessment rather than treating any single observation as conclusive.
It supports construction and evaluation of blood-brain barrier models, drug transport studies, and vascular tissue engineering. Researchers can also use characterization to examine how neurovascular properties change during development, injury, inflammation, or neurological disease. The resulting profiles help connect endothelial phenotype and barrier behavior with vascular contributions to neural tissue function.