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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Article DOI: 10.3791/55567-v • 09:58 min • May 2nd, 2017
May 2nd, 2017 •
1, 1, 2, 3, 4, 1, 1
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, 2Physikalisch-Technische Bundesanstalt, 3Department of Radiology and Neuroradiology, Ernst-Moritz-Arndt-University Greifswald, 4Electron Microscopy Center, University of Rostock

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Summary
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This manuscript describes the efficient, non-viral delivery of miR to endothelial cells by a PEI/MNP vector and their magnetization. Thus, in addition to genetic modification, this approach allows for magnetic cell guidance and MRI detectability. The technique can be used to improve the characteristics of therapeutic cell products.

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Preparation In Vitro Characterization Magnetized MiR-modified Endothelial Cells Technique Cell Modification MicroRNA Cell Magnetization Regenerative Medicine Field Genetic Engineering Transplantation Cell Retention Magnetic Field Application Cell Therapy Heart Diseases Hypocell Retention Cell Survival Human Umbilical Vein Endothelial Cell Culture Transfection Complexes HUVECs Plastic Cell Culture Well Plates Starting Cell Density MicroRNA/polyethyleneimene/superparamagnetic Iron Oxide Nanoparticles Fresh MiR Solution Glucose Solution PEI Dilution
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