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Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
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Chapters
- 00:05Title
- 00:58Endothelial Cell Culture and Tissue Culture Conditions
- 02:42Evaluate Endothelial Factor Released on Leukocyte Activation by Adhesion Assays
- 04:12Leukocyte Adhesion Assay
- 07:42Test Endothelial Activation by Leukocyte Endothelium Co-adhesion Assay
- 09:59Results: Activation Parameters of LPS-stimulated Endothelial Cells and Influence on Leukocyte Behavior
- 11:52Conclusion
Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.
