Live Animal Imaging
Live Animal Imaging - Related Videos
01:37
Non-invasive Monitoring and Imaging in Animal Research
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Non-invasive technologies support the principles of 3Rs by enabling repeated data collection from the same animal without the need for euthanasia at multiple time points. These approaches reduce the number of animals required...
08:05
Doppler Ultrasonography for Live Imaging and Quantification of Ovarian Vascular Function in Mice
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Cited by 1 •
2025
Changes in ovarian blood flow during the preovulatory period are essential for ovulation. Doppler ultrasonography has been used in humans and large animals to assess ovarian hemodynamics and perfusion. A protocol is presented for applying Doppler ultrasonography to evaluate ovarian hemodynamics during the preovulatory and periovulatory phases in mice.
09:57
Live Cell Imaging of Mitosis
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2023
Mitosis is a form of cell division in which a cell’s genetic material is divided equally between two daughter cells. Mitosis can be broken down into six phases, during each of which the cell’s components, such as its chromosomes, show visually distinct characteristics. Advances in fluorescence live cell imaging have allowed scientists to study this process in great detail, providing important insights into the biological control of this process and how it might go wrong in diseases such as...
26:43
Computer-Generated Animal Model Stimuli
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Cited by 8 •
2007
Computer-generated stimuli using the Jacky dragon as a model.
07:42
Live Cell Imaging during Mechanical Stretch
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Cited by 12 •
2015
A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.
FAQs
Each modality produces contrast through a different signal source. Fluorescence microscopy detects signals from fluorescent probes, whereas magnetic resonance imaging relies on magnetic properties, and positron emission tomography maps radiotracer distribution. These signals provide complementary views of neuronal activity, brain connectivity, blood flow, metabolism, or disease-related change. The choice determines which biological process can be measured and compared over time.
No single imaging approach optimizes every experimental requirement. Higher structural or activity detail may come with greater sensitivity to motion or increased invasiveness, while a biologically relevant signal may not be highly specific to one process. Researchers therefore match the method to the question, weighing how clearly it captures the target against how well it preserves physiological conditions for repeated measurements.
Repeated measurements allow researchers to follow changes in the same living organism rather than relying only on separate tissue samples collected at different time points. This design can reveal trajectories associated with learning, neurodegeneration, or therapeutic response. Preserving physiological context also helps connect changing brain signals with behavior, making temporal relationships easier to examine.
A suitable strategy begins by identifying the process of interest, such as neuronal activity, connectivity, blood flow, metabolism, or disease progression. Researchers then select a signal source that can represent that process and assess practical constraints, including resolution, invasiveness, motion, and signal specificity. This alignment helps ensure that the resulting measurements address the biological question rather than merely producing images.
The approach supports studies that require brain measurements alongside ongoing biological or behavioral change. Applications described for neuroscience include examining learning, tracking neurodegeneration, and evaluating therapeutic response. Because measurements can be collected while physiological context is retained, researchers can relate imaging changes to behavior and assess how neural or disease-associated patterns evolve over time.
Researchers can compare imaging-derived measures with behavioral changes in the same living organism. For example, signals related to neuronal activity, connectivity, blood flow, or metabolism may be examined alongside learning-related behavior or disease progression. This pairing helps place brain measurements in functional context, while the selected modality determines which aspect of neural or physiological change is most directly represented.