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Journal / Bioengineering / Live Cell Imaging during Mechanical Stretch
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Live Cell Imaging during Mechanical Stretch

Article DOI: 10.3791/52737-v • 07:43 min • August 19th, 2015
August 19th, 2015 •
1,2, 3, 4, 1,2, 1,2, 3
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Biomedical Engineering and Imaging, University of Tennessee Health Science Center, 3Department of Biomedical Engineering, University of Memphis, 4Department of Engineering Technology, University of Memphis

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Summary
Automatic Translation

A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.

Tags

Live Cell Imaging Mechanical Stretch Cells And Tissues Mechanical Stimuli Real-time Measurement Live Cells Viable Tissue Cell Actuator Distend Live Cells Elastic Substrate Confocal Microscopy Atomic Force Microscopy (AFM) Bronchial Epithelial Cells Mitochondrial Superoxide Production Alveolar Epithelial Cells Lung Injury In Vitro AFM Nano-indentation
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