Lung Perfusion Fixation
Lung Perfusion Fixation - Related Videos
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
07:19
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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Cited by 3 •
2021
Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.
06:57
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Cited by 48 •
2015
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
05:35
Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume
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2023
This video describes a simple and reliable method of introducing liquid into the trachea for lung instillation, fixation, and lung volume measurement.
FAQs
The circulating fixative reaches lung tissue through the pulmonary vasculature after displacing blood. This vascular delivery helps stabilize structures throughout the specimen rather than leaving regions vulnerable to postmortem degradation. As a result, microscopic analysis can more reliably examine the spatial relationships among alveolar or other lung cells, airways, and blood vessels.
Blood displacement reduces the barrier between the fixative and vascularly accessible pulmonary tissues. It allows those tissues to receive the stabilizing solution and limits changes that could occur as the specimen degrades after death. This improves the preservation of tissue organization, which is essential when comparing vascular, airway, and cellular features microscopically.
Consistent fixation produces specimens with more comparable structural preservation across experimental conditions. That consistency supports clearer histological assessment and reduces the likelihood that differences in tissue appearance reflect uneven preservation rather than biology. It also strengthens anatomical comparisons by maintaining the relative organization of cells, airways, and blood vessels for microscopic evaluation.
Preserved pulmonary architecture provides a structural basis for investigating brain–lung interactions and respiratory control. It can also help researchers examine neuroimmune signaling or neural influences on pulmonary tissue when those questions require tissue-level anatomical analysis. Maintaining relationships among pulmonary structures makes it easier to relate observed changes to broader nervous-system and lung interactions.
The central workflow is to circulate a fixative solution through the pulmonary vasculature, allow it to displace blood, and expose vascular tissues to the fixative. The resulting stabilized specimen can then undergo microscopic evaluation. This sequence is important because vascular delivery connects the preservation step directly to the lung’s internal structural organization.
Lung Perfusion Fixation supports several microscopy-based analyses, including histology and immunolabeling. It also enables anatomical comparison among specimens or experimental conditions by preserving tissue structure and spatial relationships. These outcomes are useful when researchers need to evaluate pulmonary changes alongside neural, respiratory, or neuroimmune questions in neuroscience studies.
This approach is useful when a neuroscience experiment examines how neural activity or signaling relates to pulmonary structure. Relevant applications include studies of brain–lung interactions, respiratory control, neuroimmune signaling, and neural influences on lung tissue. Preserved anatomy can connect these functional or signaling questions with observable changes in cells, airways, and blood vessels.