Lung Tissue Extraction
Lung Tissue Extraction - Related Videos
03:03
Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs
0 Views •
2025
In this video, we describe a surgical procedure to extract bronchiolar tissues from harvested pig lung. The ex vivo bronchiolar tissue could be used to develop infection models for studying chronic lung infections.
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
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2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
08:32
Extraction of Tissue Antigens for Functional Assays
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2012
A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.
11:10
Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
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Cited by 127 •
2011
The stiffness of the extracellular matrix strongly influences multiple behaviors of adherent cells. Matrix stiffness varies spatially throughout a tissue, and undergoes modification in various disease conditions. Here we develop methods to characterize spatial variations in stiffness in normal and fibrotic mouse lung tissue using atomic force microscopy microindentation.
03:48
Mouse Lung Preparation for Flow Cytometry: Generating Cell Suspension from Lung Tissue for Flow Cytometric Analysis
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2023
This video describes the preparation of mouse lung cell suspension for flow cytometric analysis to study PD-L1 expression of lung adenocarcinoma cells.
FAQs
Immediate preservation helps maintain the lung sample’s tissue architecture and biological molecules after removal from the organism. This is essential because later analyses may depend on intact structural relationships, cellular features, or molecular content. The chosen handling approach should therefore match the planned examination, whether the goal is histology, microscopy, molecular assays, or cell-based analysis.
Careful removal of surrounding tissues isolates the lung material needed for analysis and supports clearer interpretation of the sample. Dissection must balance separation with preservation of the lung’s structure. Well-prepared tissue can retain the anatomical and cellular features required to examine pulmonary changes and their possible relationship to neural or systemic processes.
The intended analysis determines how the extracted material should be preserved or processed. Structural studies emphasize maintaining tissue architecture, whereas molecular assays require preservation of biological molecules. Cellular methods may require handling that supports examination of cells within the sample. Matching preparation to the analytical goal improves the relevance of the resulting observations.
Lung samples can provide material for investigating neuroimmune signaling, pulmonary influences on nervous system function, and communication between respiratory tissues and neural pathways. These studies place lung biology within a broader systemic context rather than treating the tissue as an isolated organ. Findings may help connect pulmonary physiology or pathology with aspects of neural health.
The procedure generally proceeds through careful dissection, removal of surrounding tissues, and immediate preservation or processing. Each stage contributes to obtaining a usable sample while maintaining relevant structural and biological features. After preparation, the material can be directed toward histology, microscopy, molecular assays, or cell-based methods according to the research question.
The choice depends on what the sample must reveal. Preservation is appropriate when investigators need to maintain tissue architecture or biological molecules for later examination, while immediate processing may support workflows that require prompt access to the extracted material. In either case, handling should be planned around the intended structural, molecular, or cellular analysis.
Histology and microscopy can support assessment of tissue structure and cellular organization, while molecular assays can examine biological molecules. Cell-based methods provide another route for studying material derived from the sample. In neuroscience-related work, these outcomes can be interpreted alongside questions about pulmonary pathology, neuroimmune signaling, and respiratory interactions with neural pathways.