Extraction of Tissue Antigens for Functional Assays

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Extraction of Tissue Antigens for Functional Assays

DOI:

10.3791/4230-v

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08:32 min

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September 10, 2012

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Andra Necula, Rochna Chand, Batool Albatat, Stuart I. Mannering²

¹Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, ²Department of Medicine,University of Melbourne

Chapters

00:05Title
01:13Preparing Spleen Tissue
01:57Preparing Human Islets for Storage
02:43Preparing Tissue Extract
04:47Freeze Drying Extracts
06:17Results: Functional T cell Assays of Extracts
08:08Conclusion

Summary

Automatic Translation

A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.

Extraction of Tissue Antigens for Functional Assays

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