Miniscope Imaging
Miniscope Imaging - Related Videos
02:55
Real-Time Calcium Imaging of a Mouse Medial Prefrontal Cortex Using a Miniscope
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2025
Source: Thapa, R., et. al., Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging. J. Vis. Exp. (2021)The video demonstrates real-time calcium imaging using a miniscope in a transduced mouse, correlating neuronal activity with behavior.
06:21
Miniscope Recording Calcium Signals at Hippocampus of Mice Navigating an Odor Plume
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Cited by 2 •
2024
This protocol investigates the brain-behavior relationship in hippocampal CA1 in mice navigating an odor plume. We provide a step-by-step protocol, including surgery to access imaging of the hippocampus, behavioral training, miniscope GCaMP6f recording and processing of the brain, and behavioral data to decode the mouse position from ROI neural activity.
06:23
Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice
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Cited by 4 •
2021
The shrinkage of dental cement during curing displaces the baseplate. This protocol minimizes the problem by creating an initial foundation of the dental cement that leaves space to cement the baseplate. Weeks later, the baseplate can be cemented in position on this scaffold using little new cement, therebyreducing shrinkage.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
FAQs
The system tracks changes in fluorescence emitted by genetically encoded calcium indicators or other fluorescent reporters. These changes are recorded over time and converted into activity signals associated with identified neurons. This time-resolved readout allows researchers to examine when particular cells become active during behaviors such as navigation, learning, or social interaction.
Fluorescent reporters provide the light signal linked to neural activity, while an implanted optical interface, such as a GRIN lens, allows the head-mounted microscope to detect that signal from the brain. Together, these components connect cellular activity with ongoing behavior and make it possible to monitor defined neuronal populations during freely moving experiments.
Its head-mounted format allows animals to move more naturally during recording rather than remaining confined to a head-fixed preparation. This distinction is important when movement, exploration, social interaction, or other behavior-related factors could influence neural activity. The resulting measurements can relate brain-cell dynamics to behavior in settings that better preserve natural movement.
A typical workflow combines a fluorescent reporter with an implanted optical interface and a lightweight microscope worn by the animal. The microscope records fluorescence while the animal performs a behavioral task or moves freely. Researchers then examine the time-resolved signals from identified cells in relation to the observed behavior and experimental condition.
Following the same cells across multiple experiences allows researchers to examine how neural activity changes over time rather than comparing unrelated cell populations at separate time points. This capability supports studies of learning, navigation, social interaction, and disease-related behaviors, including how cellular responses evolve as animals encounter new conditions or repeated experiences.
The method can reveal how distributed neural circuits encode behavior by examining activity across identified cells during natural actions. Researchers can compare these patterns across experiences or experimental conditions, helping connect cellular signals with broader circuit-level organization. Such analyses are relevant to behavioral studies and to investigations of disease-related changes in neural function.