Acute In Vivo Recording
Acute In Vivo Recording - Related Videos
07:37
Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings
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Cited by 3 •
2021
This protocol describes a procedure for constructing carbon fiber microelectrode arrays for chronic and acute in vivo electrophysiological recordings in mouse (Mus musculus) and ferret (Mustela putorius furo) from multiple brain regions. Each step, following the purchase of raw carbon fibers to microelectrode array implantation, is described in detail, with emphasis on microelectrode array construction.
10:46
Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats
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Cited by 13 •
2017
In this study, the methodology is presented on how to perform multi-site in vivo electrophysiological recordings from the hyperdirect pathway under urethane anesthesia.
16:38
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
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Cited by 19 •
2012
The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.
07:10
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
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Cited by 25 •
2013
We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.
12:32
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
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Cited by 14 •
2013
Retrograde transport of fluorescent dye labels a sub-population of neurons based on anatomical projection. Labeled axons can be visually targeted in vivo, permitting extracellular recording from identified axons. This technique facilitates recording when neurons cannot be labeled through genetic manipulation or are difficult to isolate using 'blind' in vivo approaches.
FAQs
The recorded signal depends on which neural events are being examined. Action potentials provide electrical evidence associated with individual neuronal firing, whereas local field potentials reflect activity measured across a broader local circuit. Recording both types can connect fast cellular events with surrounding network dynamics, helping investigators distinguish moment-to-moment firing from population-level activity.
Targeted probe placement determines which brain region contributes to the dataset and therefore shapes the circuit question being tested. Electrodes or other recording probes must be positioned at the region of interest so detected activity can be related to sensory input, behavior, stimulation, or disease-related change. This targeting gives measurements anatomical meaning within the nervous system.
Amplification and digitization convert biological signals detected by the probe into data suitable for analysis. The recording system strengthens the measured signals and converts them into digital information that can be examined over time. This processing preserves the timing information needed to study fast neural events and circuit dynamics, making the measurements usable for quantitative comparisons.
Acute in vivo recording measures activity while the animal’s nervous system remains in its natural biological context, whereas ex vivo approaches examine tissue outside the intact organism. The in vivo approach therefore supports links between neural signals and sensory input, behavior, stimulation, or disease-related changes. It complements rather than replaces ex vivo experiments, which address neural activity under different conditions.
During a short experimental session, researchers select a neural target, position electrodes or another probe, collect electrical signals, and pass them through amplification and digitization before analysis. The workflow can be organized around a sensory input, behavior, stimulation, or disease-related condition, allowing recorded activity to be interpreted alongside the relevant experimental event.
Researchers use this approach when they need to relate neural activity to events occurring in a living animal. It can reveal how circuits respond during sensory processing, behavior, or stimulation, and it can help characterize disease-related changes. Because measurements occur in an intact biological context, the data support circuit-level interpretations that isolated tissue does not directly capture.