Mitotracker Deep Red
Mitotracker Deep Red - Related Videos
01:23
Red Algae
0 Views •
2025
Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
09:45
In vivo Imaging of Deep Cortical Layers using a Microprism
0 Views •
Cited by 11 •
2009
Right-angle microprisms inserted into the mouse neocortex allows for deep imaging of multiple cortical layers with a viewpoint typically found in slice. One-millimeter microprisms offer a wide field-of-view (~900 μm) and spatial resolutions sufficient to resolve dendritic spines. We demonstrate layer V neuronal imaging and neocortical vascular imaging using microprisms.
05:44
Using Saccadometry with Deep Brain Stimulation to Study Normal and Pathological Brain Function
0 Views •
Cited by 7 •
2016
This paper describes the use of quantitative measurement of eye movements in conjunction with stimulation of focal areas of the deep brain in order to study physiology, pathophysiology, and the mechanisms of deep brain stimulation.
11:09
Deep Brain Stimulation with Simultaneous fMRI in Rodents
0 Views •
Cited by 21 •
2014
This protocol describes a standard method for simultaneous functional magnetic resonance imaging and deep brain stimulation in the rodent. The combined use of these experimental tools allows for the exploration of global downstream activity in response to electrical stimulation at virtually any brain target.
09:12
Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
0 Views •
Cited by 51 •
2018
Here we present techniques to measure red cell deformability and cellular heterogeneity by ektacytometry. These techniques are applicable to general investigations of red cell deformability and specific investigations of blood diseases characterized by the presence of both rigid and deformable red cells in circulation, such as sickle cell anemia.
FAQs
Membrane potential determines much of the initial mitochondrial labeling: organelles with an intact potential preferentially accumulate the cell-permeant dye. Consequently, fluorescence patterns should be interpreted in relation to mitochondrial functional state, not only organelle location. In neuronal experiments, this feature helps researchers examine how changes associated with stress or injury coincide with altered mitochondrial organization.
The reactive chloromethyl group promotes retention of the fluorescent signal within mitochondrial structures after staining. This retention is important because it allows labeled mitochondria to remain trackable during live-cell imaging rather than limiting observation to the moment of dye exposure. It therefore supports analysis of mitochondrial distribution, morphology, and movement in neuronal cells.
Changes in the observed pattern can be examined at several organizational levels: mitochondrial distribution within a cell, morphology of individual structures, and their dynamics over time. In neurons, the same labeling strategy can reveal whether mitochondria are positioned in cell bodies, axons, dendrites, or synaptic regions, linking spatial organization to neuronal function.
Combining MitoTracker Deep Red with other neuronal markers adds cellular context that the mitochondrial signal alone cannot provide. Co-localization can help identify whether labeled mitochondria occupy neuronal cell bodies, axons, dendrites, or synapses. This approach supports interpretation of mitochondrial placement alongside neuronal structure, rather than treating fluorescence as an isolated readout.
A basic workflow pairs live-cell labeling with fluorescence microscopy, then examines labeled mitochondrial patterns in the relevant neuronal compartments. Because the dye is cell-permeant and signal retention occurs after staining, researchers can follow structures in living cells. Imaging can be complemented by neuronal markers to assign mitochondrial signals to specific cellular locations.
In neuroscience, the technique is particularly useful for studying mitochondrial transport along axons and dendrites. Imaging can show how mitochondria are distributed across these processes and where they appear near synaptic regions. Such observations help connect organelle positioning with the energy supply and functional organization of neurons.
Researchers can apply the labeling strategy to cellular stress or injury models to examine accompanying changes in mitochondrial distribution, morphology, or dynamics. The resulting images do not simply describe where mitochondria are; they provide a way to relate their organization and membrane-potential-dependent labeling to processes relevant to neurodegeneration and neuronal function.