Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

David R. Myers; Yumiko Sakurai; Reginald Tran; Byungwook Ahn; Elaissa Trybus Hardy; Robert Mannino; Ashley Kita; Michelle Tsai; Wilbur A. Lam

doi:10.3791/3958

Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Endothelialized على microfluidics لدراسة التفاعلات الاوعية الدموية الدقيقة في الأمراض الدموية

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11:08 min

June 22, 2012

DOI: 10.3791/3958-v

David R. Myers*^1,2,3,4, Yumiko Sakurai*^1,2,3,4, Reginald Tran^1,2,3,4, Byungwook Ahn^1,2,3,4, Elaissa Trybus Hardy^1,2,3,4, Robert Mannino^1,2,3,4, Ashley Kita^1,2,3,4, Michelle Tsai^1,2,3,4, Wilbur A. Lam^1,2,3,4

¹Department of Pediatrics,Emory University School of Medicine, ²Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, ³Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta , ⁴Winship Cancer Institute of Emory University

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Overview

This article describes a method to culture an endothelial cell monolayer in a microfluidic device with microvascular-sized channels. This in vitro model facilitates the study of interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.

Key Study Components

Area of Science

Microfluidics
Cell culture
Hematologic diseases

Background

Endothelial cells play a crucial role in vascular biology.
Microfluidic devices allow for precise control of the cellular environment.
Studying cell interactions in vitro can provide insights into disease mechanisms.
This method aims to replicate the microvascular environment.

Purpose of Study

To develop a microfluidic device coated with endothelial cells.
To enable the study of cell-to-cell interactions.
To provide a platform for investigating hematologic diseases.

Methods Used

Fabrication of the microfluidic mold.
Creation of PDMS channels.
Seeding of endothelial cells into the device.
Culturing the cells within the device.

Main Results

Successful creation of endothelial cell monolayers.
Demonstration of cell-to-cell interactions in vitro.
Potential applications in studying hematologic conditions.
Validation of the microfluidic model for future research.

Conclusions

The method provides a reliable platform for endothelial cell studies.
It enhances understanding of biophysical interactions in blood-related diseases.
Future research can leverage this model for therapeutic insights.

Frequently Asked Questions

What is the significance of endothelial cells?

Endothelial cells line blood vessels and are crucial for vascular function and health.

How does microfluidics contribute to cell culture?

Microfluidics allows for precise control of the cellular environment, enabling more accurate studies.

What diseases can be studied using this model?

This model can be used to study various hematologic diseases and their mechanisms.

What materials are used in the microfluidic device?

The device is primarily made from PDMS, a silicone elastomer commonly used in microfluidics.

Can this method be applied to other types of cells?

Yes, the method can potentially be adapted for other cell types to study different interactions.

What are the advantages of using an in vitro model?

In vitro models allow for controlled experiments and can mimic physiological conditions without the ethical concerns of in vivo studies.

< p class = "jove_content" > تم وصف طريقة لزراعة طبقة أحادية الخلية البطانية في جميع أنحاء السطح الداخلي ثلاثي الأبعاد بالكامل لجهاز موائع دقيقة مع قنوات بحجم الأوعية الدموية الدقيقة (<30 ميكرومتر). يتيح نموذج الأوعية الدموية الدقيقة في المختبر دراسة التفاعلات الفيزيائية الحيوية بين خلايا الدم والخلايا البطانية والعوامل القابلة للذوبان في أمراض الدم.

الهدف العام من هذا الإجراء هو إنشاء جهاز ميكروفيليديك مغطى بالخلايا البطانية. يتم تحقيق ذلك عن طريق تصنيع قالب الموائع الدقيقة أولا. الخطوة الثانية هي إنشاء قناة PDMS.

بعد ذلك ، يتم زرع الخلايا البطانية في الجهاز. الخطوة الأخيرة هي زراعة الخلايا داخل الجهاز. في النهاية ، يتم استخدام أجهزة الموائع الدقيقة المطلية بالخلايا البطانية لإظهار تفاعلات الخلية إلى الخلية في المختبر.

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