المعدلة وراثيا القوارض الفحص لقياس ذكر جرثومة خلية متحولة التردد

2:30

Exposures, Isolation, and Digestion of Cauda Sperm and DNA Extraction

6:45

Day Before Assay: Preparation of Agar and Overnight Culture

7:43

LacZ Mutation Assay: Sub-culture Cells

8:43

LacZ Mutation Assay: Packaging DNA in Lambda Phage Particles and Preparation of Top Agar

10:36

LacZ Mutation Assay: Infection of Cells and Counting Plaques

12:40

Results: Measurement of Mutant Frequency in Transgenic Mice Cauda Sperm

13:36

Conclusion