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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Immunology and Infection
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JoVE Journal Immunology and Infection
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

DOI:
10.3791/51492-v

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12:55 min

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January 17, 2015

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, , , , , , , , , , ,
1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research,University of Toronto, 3Antibiome Center,University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology,The University of Chicago

Chapters

  • 00:05Title
  • 02:17Preparing Cells for Phage Selection
  • 03:28Library Pre-clearance and Antigen-phage Incubation
  • 05:45Phage Elution and Amplification
  • 08:03Characterization by Pooled ELISA
  • 10:22Anticipated Results and Troubleshooting
  • 12:18Conclusion

Summary

Automatic Translation

Automatic Translation

A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.

Tags

Keywords: Phage DisplaySynthetic Antibody LibrariesHigh-throughput SelectionFab FragmentsAntigen CharacterizationAntibody DiscoveryImmunoassays

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