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JoVE Journal
Immunology and Infection

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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
 

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Article doi: 10.3791/51492
January 17th, 2015 Usage Statistics

Summary January 17th, 2015

A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.

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