renal-ischaemia-reperfusion-injury-mouse-model-injury

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration

Renal ischaemia reperfusion injury (IRI) is a common cause of acute kidney injury (AKI) in patients and occlusion of renal blood flow is unavoidable ...

mouse-kidney-transplantation-models-of-allograft-rejection

Mouse Kidney Transplantation: Models of Allograft Rejection

Rejection of the transplanted kidney in humans is still a major cause of morbidity and mortality. The mouse model of renal transplantation closely ...

a-murine-model-irreversible-reversible-unilateral-ureteric

A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction

Obstruction of the kidney may affect native or transplanted kidneys and results in kidney injury and scarring. Presented here is a model of obstructive ...

University of Edinburgh

The University of Edinburgh

Large-scale RNAi-based screens are a major technology, but require adequate prioritization and validation of candidate genes from the primary screen. In this work, we performed a large-scale pooled shRNA screen in mouse embryonic stem cells (ESCs) to discover genes associated with oxidative stress resistance and found several candidates. We then developed a bioinformatics pipeline to prioritize these candidates incorporating effect sizes, functional enrichment analysis, interaction networks and gene expression information. To validate candidates, we mixed normal cells with cells expressing the shRNA coupled to a fluorescent protein, which allows control cells to be used as an internal standard, and thus we could detect shRNAs with subtle effects. Although we did not identify genes associated with oxidative stress resistance, as a proof-of-concept of our pipeline we demonstrate a detrimental role of Edd1 silencing in ESC growth. Our methods may be useful for candidate gene prioritization of large-scale RNAi-based screens.

An analysis and validation pipeline for large-scale RNAi-based screens.

Acute kidney injury induced by renal ischaemia reperfusion injury (IRI) is characterised by renal failure, acute tubular necrosis (ATN), inflammation and microvascular congestion. The administration of apoptotic cells (ACs) has been shown to reduce inflammation in various organs including the liver and kidney. This study explored whether AC administration prior to the induction of renal IRI was protective.

ArticleTotal : 3	Year
Publication title Cited by 110	2014
Publication title Cited by 17	2014
Publication title Cited by 47	2014

Article	Year
An analysis and validation pipeline for large-scale RNAi-based screens. Scientific reports\| PubMed ID: 23326633	2013
Apoptotic cell administration is detrimental in murine renal ischaemia reperfusion injury. Journal of inflammation (London, England)\| PubMed ID: 25317079	2014

Emily E. Hesketh

Biography

JoVE Journal Publications

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