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JoVE Journal Medicine
Mouse Kidney Transplantation
Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix
Journal (Medicine)
Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix
Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
Journal (Medicine)
Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
 

Mouse Kidney Transplantation: Models of Allograft Rejection

Article DOI: 10.3791/52163
October 11th, 2014 •
*1, *1, 1, 1, 1, 1
1MRC Centre for Inflammation Research, The Queen's Medical Research Institute, The University of Edinburgh
* These authors contributed equally

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October 11th, 2014

Here, we present a protocol to study the immunology of rejection. The surgical model presented reports a short operating time and a concise technique. Depending on the donor-recipient strain combination, the transplanted kidney may develop acute cellular rejection or chronic allograft damage, defined by interstitial fibrosis and tubular atrophy.

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