Mouse Kidney Transplantation: Models of Allograft Rejection

This video presents a mouse model of kidney transplantation. By utilizing the multiple inbred strains, which have known major histocompatibility classes we're able to recreate different aspects of rejection of the renal allograft. The presented data illustrates how this is a useful model In the study of these mechanisms, This Schematic cartoon demonstrates the major steps of this model.

A left nephrectomy is performed on a donor mouse and the donor kidney is then prepared on a sterile swab. In a culture dish, the recipient mouse undergoes a right nephrectomy before The donor kidney is transplanted. The procedure is performed using sterile autoclaved instruments and consumables positioned within a sterile surgical field.