osteoclast-derivation-from-mouse-bone-marrow

Osteoclast Derivation from Mouse Bone Marrow

Osteoclasts are highly specialized cells that are derived from the monocyte/macrophage lineage of the bone marrow. Their unique ability to resorb both the ...

isolation-enrichment-human-adipose-derived-stromal-cells-for-enhanced

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are considered the gold standard for stem cell-based tissue engineering applications. However, the ...

assessment-viability-human-fat-injection-into-nude-mice-with-micro

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Lipotransfer is a vital tool in the surgeon&#8217;s armamentarium for the treatment of soft tissue deficits of throughout the body. Fat is the ideal soft ...

rapid-isolation-bmpr-ib-adipose-derived-stromal-cells-for-use

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

Invasive cancers, major injuries, and infection can cause bone defects that are too large to be reconstructed with preexisting bone from the patient's own ...

Stanford University School of Medicine

For more than a century, colorimetric and fluorescence staining have been the foundation of a broad range of key bioanalytical techniques. The dynamics of such staining processes, however, still remains largely unexplored. We investigated the kinetics of fluorescence staining of two gram-negative and two gram-positive species with 3,3'-diethylthiacyanine (THIA) iodide. An increase in the THIA fluorescence quantum yield, induced by the bacterial dye uptake, was the principal reason for the observed emission enhancement. The fluorescence quantum yield of THIA depended on the media viscosity and not on the media polarity, which suggested that the microenvironment of the dye molecules taken up by the cells was restrictive. The kinetics of fluorescence staining did not manifest a statistically significant dependence neither on the dye concentration, nor on the cell count. In the presence of surfactant additives, however, the fluorescence-enhancement kinetic patterns manifested species specificity with statistically significant discernibility.

Kinetics of bacterial fluorescence staining with 3,3'-diethylthiacyanine.

The ability of the human cranium to ossify full-thickness defects depends on the size of the area and the age of the patient. An adult leporid cranioplasty model is commonly used to study inlay cranioplasty materials; the influence of age on ossification is unknown in this model. The purpose of this study was to determine the effect of age on healing of a rabbit critical-size defect. Nineteen rabbits were divided into 4 groups: group 1 (n = 5) aged 4 months, group 2 (n = 4) aged 8 months, group 3 (n = 5) aged 12 months, and group 4 (n = 5) aged 16 months. A 17 × 17-mm defect was created in the parietal bones with preservation of the underlying dura. Animals underwent micro-computed tomography 4 months postoperatively to determine ossification of the defect. Group 1 defects healed by 28.5% (SD, 12.5%), group 2 defects ossified by 37.2% (SD, 5.7%), group 3 defects closed by 28.2% (SD, 11.9%), and group 4 defects healed by 39.4% (SD, 11.0%). No difference in ossification was found between groups (P = 0.31).Leporids as young as 4 months do not close a 17 × 17-mm defect; ossification is similar to animals as old as 16 months. Rabbits 4 months or older are suitable for a calvarial critical-size defect model.

Critical-size defect ossification: effect of leporid age in a cranioplasty model.

Fat grafting has become increasingly popular for the correction of soft-tissue deficits at many sites throughout the body. Long-term outcomes, however, depend on delivery of fat in the least traumatic fashion to optimize viability of the transplanted tissue. In this study, the authors compare the biological properties of fat following injection using two methods.

Studies in fat grafting: Part I. Effects of injection technique on in vitro fat viability and in vivo volume retention.

Early fetuses heal wounds without the formation of a scar. Many studies have attempted to explain this remarkable phenomenon. However, the exact mechanism remains unknown. Herein, we examine the predominant cell types of the epidermis and dermis--the keratinocyte and fibroblast--during different stages of fetal development to better understand the changes that lead to scarring wound repair versus regeneration.

Gene expression in fetal murine keratinocytes and fibroblasts.

Although fat grafting can address many soft-tissue deficits, results remain inconsistent. In this study, the authors compared physical properties of fat following injection using an automated, low-shear device or the modified Coleman technique.

Studies in fat grafting: Part II. Effects of injection mechanics on material properties of fat.

Adipose derived stromal cells obtained by ultrasound assisted liposuction versus suction assisted liposuction: a comparison of their regenerative capacity.

Key transcriptional differences exist in diabetic fracture healing.

What Makes a Plastic Surgery Residency Attractive: An Applicant's Perspective.

Wounds, both chronic and acute, continue to be a tremendous socioeconomic burden. As such, technologies drawn from many disciplines within science and engineering are constantly being incorporated into innovative wound healing therapies. While many of these therapies are experimental, they have resulted in new insights into the pathophysiology of wound healing, and in turn the development of more specialized treatments for both normal and abnormal wound healing states. Herein, we review some of the emerging technologies that are currently being developed to aid and improve wound healing after cutaneous injury.

ArticleTotal : 4	Year
Publication title Cited by 30	2014
Publication title Cited by 11	2015
Publication title Cited by 8	2015
Publication title Cited by 1	2017

Article	Year
Kinetics of bacterial fluorescence staining with 3,3'-diethylthiacyanine. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 20481488	2010
Critical-size defect ossification: effect of leporid age in a cranioplasty model. The Journal of craniofacial surgery\| PubMed ID: 22134272	2011
Studies in fat grafting: Part I. Effects of injection technique on in vitro fat viability and in vivo volume retention. Plastic and reconstructive surgery\| PubMed ID: 24622574	2014
Gene expression in fetal murine keratinocytes and fibroblasts. The Journal of surgical research\| PubMed ID: 24726057	2014
Studies in fat grafting: Part II. Effects of injection mechanics on material properties of fat. Plastic and reconstructive surgery\| PubMed ID: 25028817	2014
Adipose derived stromal cells obtained by ultrasound assisted liposuction versus suction assisted liposuction: a comparison of their regenerative capacity. Plastic and reconstructive surgery\| PubMed ID: 25254755	2014
Key transcriptional differences exist in diabetic fracture healing. Plastic and reconstructive surgery\| PubMed ID: 25254763	2014
What Makes a Plastic Surgery Residency Attractive: An Applicant's Perspective. Plastic and reconstructive surgery\| PubMed ID: 25254766	2014
Wound healing: an update. Regenerative medicine\| PubMed ID: 25431917	2014

Elizabeth R. Zielins

Biography

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