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Developmental Biology

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Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
 

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Article doi: 10.3791/52181
January 12th, 2015

Summary January 12th, 2015

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

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