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Journal / Biology / Osteoclast Derivation from Mouse Bone Marrow
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JoVE Journal Biology
Osteoclast Derivation from Mouse Bone Marrow
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Osteoclast Derivation from Mouse Bone Marrow

Article DOI: 10.3791/52056-v • 06:17 min • November 6th, 2014
November 6th, 2014 •
*1, *1,2, 2, 2, 1,2, 1,2, 1,2, 1, 1, 1, 1, 1, 1, 1,2, 1,2
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University
* These authors contributed equally

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Summary
Automatic Translation

Osteoclasts are the principal bone-resorbing cell in the body. An ability to isolate osteoclasts in large numbers has resulted in significant advances in the understanding of osteoclast biology. In this protocol, we describe a method for isolation, cultivating and quantifying osteoclast activity in vitro.

Tags

Osteoclast Derivation Mouse Bone Marrow Monocyte/macrophage Lineage Bone Resorption Skeletal Remodeling Osteoblasts Bone Formation Osteoclast Differentiation Osteoclastic Bone Resorption Hematopoiesis Osteoporosis Fracture Formation Isolate Osteoclasts In Vitro Cultivation Therapeutic Strategies
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