Journal
/
Biology
/
Osteoclast Derivation from Mouse Bone Marrow
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Osteoclast Derivation from Mouse Bone Marrow

Osteoclast Derivation from Mouse Bone Marrow

DOI:
10.3791/52056-v

•

06:17 min

•

November 06, 2014

•

, , , , , , , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Chapters

  • 00:05Title
  • 01:28Media Preparation and Bone Marrow Isolation
  • 03:05Gradient Separation of Bone Marrow Cells and Cell Culturing
  • 04:57Results: Culturing Osteoclasts from Mouse Bone Marrow
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

Osteoclasts are the principal bone-resorbing cell in the body. An ability to isolate osteoclasts in large numbers has resulted in significant advances in the understanding of osteoclast biology. In this protocol, we describe a method for isolation, cultivating and quantifying osteoclast activity in vitro.

Tags

Keywords: OsteoclastBone MarrowMonocyte/macrophage LineageBone ResorptionBone RemodelingOsteoblastsSkeletal RemodelingBone MatrixBone FormationBone DiseaseOsteoporosisIn Vitro Osteoclast IsolationBone Remodeling CycleTherapeutic Strategies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52056?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/52056?article_type=v

Culture of myeloid dendritic cells from bone marrow precursors
check_url/52056?article_type=v

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
check_url/52056?article_type=v

Homing of Hematopoietic Cells to the Bone Marrow
check_url/52056?article_type=v

Isolation and Derivation of Mouse Embryonic Germinal Cells
check_url/52056?article_type=v

Derivation of Mouse Trophoblast Stem Cells from Blastocysts
check_url/52056?article_type=v

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
check_url/52056?article_type=v

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
check_url/52056?article_type=v

Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants
check_url/52056?article_type=v

In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow

Read Article