In JoVE (3)
- Cell-based Therapy for Heart Failure in Rat: Double Thoracotomy for Myocardial Infarction and Epicardial Implantation of Cells and Biomatrix
- Histological Quantification of Chronic Myocardial Infarct in Rats
- The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury
Articles by Jérémy Valentin in JoVE
Cell-based Therapy for Heart Failure in Rat: Double Thoracotomy for Myocardial Infarction and Epicardial Implantation of Cells and Biomatrix Aurélien Frobert1, Jérémy Valentin1, Stéphane Cook1, Justine Lopes-Vicente1, Marie-Noëlle Giraud1 1Cardiology, Department of Medicine, University of Fribourg Implantation of a biograft to treat myocardial infarction induced by LAD ligation in a rodent model has conventionally required two open-heart surgeries. In order to reduce mortality and provide optimal conditions for fixation of solid and gelatinous biomatrices associated with cells, minimally invasive procedures have been developed.
Histological Quantification of Chronic Myocardial Infarct in Rats Jérémy Valentin1, Aurélien Frobert1, Guillaume Ajalbert1, Stéphane Cook1, Marie-Noëlle Giraud1 1Cardiology, Department of Medicine, University of Fribourg The post-mortem assessment of myocardial infarction (MI) in rodents is based on quantification of the infarct on stained heart sections. We describe an accurate method to quantify the infarct size using systematic sampling of harvested rat hearts from base to apex and image analyses of trichrome-stained histological sections.
The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury Manish Jain*1, Aurélien Frobert*1, Jérémy Valentin1, Stéphane Cook1, Marie-Noëlle Giraud1 1Cardiology, Department of Medicine, University of Fribourg Animal models of atherosclerosis are essential to understand the mechanism and to investigate newer approaches to prevent plaque development or rupture, a leading cause of death in the industrialized world. This protocol uses a combination of balloon injury and cholesterol rich diet to induce atherosclerotic plaques in rabbit iliac artery.
Other articles by Jérémy Valentin on PubMed
Subsurface Ablation of Atherosclerotic Plaque Using Ultrafast Laser Pulses Biomedical Optics Express. Jul, 2015 | Pubmed ID: 26203381 We perform subsurface ablation of atherosclerotic plaque using ultrafast pulses. Excised mouse aortas containing atherosclerotic plaque were ablated with ultrafast near-infrared (NIR) laser pulses. Optical coherence tomography (OCT) was used to observe the ablation result, while the physical damage was inspected in histological sections. We characterize the effects of incident pulse energy on surface damage, ablation hole size, and filament propagation. We find that it is possible to ablate plaque just below the surface without causing surface damage, which motivates further investigation of ultrafast ablation for subsurface atherosclerotic plaque removal.
Optical Coherence Tomography Findings in Bioresorbable Vascular Scaffolds Thrombosis Circulation. Cardiovascular Interventions. Oct, 2015 | Pubmed ID: 26399265 Everolimus-eluting bioresorbable vascular scaffolds have been developed to improve late outcomes after coronary interventions. However, recent registries raised concerns regarding an increased incidence of scaffold thrombosis (ScT). The mechanism of ScT remains unknown.
Prognostic Value of Troponin I for Infarct Size to Improve Preclinical Myocardial Infarction Small Animal Models Frontiers in Physiology. 2015 | Pubmed ID: 26640441 Coronary artery ligations to induce myocardial infarction (MI) in mice and rats are widely used in preclinical investigation. However, myocardial ischemic damage and subsequent infarct size are highly variable. The lack of standardization of the model impairs the probability of effective translation to the clinic. Cardiac Troponin I (cTnI) is a major clinically relevant biomarker.
Intra-Arterial Drug and Light Delivery for Photodynamic Therapy Using Visudyne®: Implication for Atherosclerotic Plaque Treatment Frontiers in Physiology. 2016 | Pubmed ID: 27672369 Photodynamic therapy (PDT), which is based on the activation of photosensitizers with light, can be used to reduce plaque burden. We hypothesized that intra-arterial photosensitizer administration and photo-activation will lead to high and rapid accumulation within the plaque with reduced systemic adverse effects. Thus, this "intra-arterial" PDT would be expected to have less side effects and due to the short time involved would be compatible with percutaneous coronary interventions.