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Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE
Microinjection of DNA Plasmid Mix into the Mouse Brain Ventricle (Video) | JoVE
Electroporation-Based DNA Plasmids Delivery to Induce In Vivo Mutations in the Mouse (Video) | JoVE
利用由常见患者突变驱动的免疫功能正常的本土肿瘤模型进行临床前测试对于免疫治疗测试至关重要。该协议描述了一种使用基于电穿孔的质粒DNA递送生成脑肿瘤小鼠模型的方法,这些质粒DNA代表常见的患者突变,从而提供准确,可重复和一致的小鼠模型。
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