In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

June 23rd, 2023

Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE

02:03 min

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Microinjection of DNA Plasmid Mix into the Mouse Brain Ventricle (Video) | JoVE

00:53 min

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Electroporation-Based DNA Plasmids Delivery to Induce In Vivo Mutations in the Mouse (Video) | JoVE

01:32 min

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Katie B. Grausam*¹, Joshua J. Breunig*^1,2,3,4,5

¹Board of Governor’s Regenerative Medicine Institute, Cedars-Sinai Medical Center, ²Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, ³Department of Biomedical Sciences, Cedars-Sinai Medical Center, ⁴Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, ⁵Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles

* These authors contributed equally

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Brug af en immunokompetent, autokton tumormodel drevet af almindelige patientmutationer til præklinisk test er kritisk for immunterapeutisk testning. Denne protokol beskriver en metode til at generere hjernetumormusemodeller ved hjælp af elektroporationsbaseret levering af plasmid-DNA, der repræsenterer almindelige patientmutationer, hvilket giver en nøjagtig, reproducerbar og konsistent musemodel.

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In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

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