In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

June 23rd, 2023

Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures (Video) | JoVE

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Microinjection of DNA Plasmid Mix into the Mouse Brain Ventricle (Video) | JoVE

00:53 min

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Electroporation-Based DNA Plasmids Delivery to Induce In Vivo Mutations in the Mouse (Video) | JoVE

01:32 min

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Katie B. Grausam*¹, Joshua J. Breunig*^1,2,3,4,5

¹Board of Governor’s Regenerative Medicine Institute, Cedars-Sinai Medical Center, ²Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, ³Department of Biomedical Sciences, Cedars-Sinai Medical Center, ⁴Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, ⁵Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles

* These authors contributed equally

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Bruk av en immunkompetent, autoktonøs tumormodell drevet av vanlige pasientmutasjoner for preklinisk testing er kritisk for immunterapeutisk testing. Denne protokollen beskriver en metode for å generere hjernesvulstmusemodeller ved hjelp av elektroporasjonsbasert levering av plasmid-DNA som representerer vanlige pasientmutasjoner, og gir dermed en nøyaktig, reproduserbar og konsistent musemodell.

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In Vivo Brain Tumor Modeling via Electroporation-Based Delivery of Plasmid DNA

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