A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

August 18th, 2023

A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings (Video) | JoVE

02:47 min

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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE

03:08 min

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Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome (Video) | JoVE

05:00 min

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Criselda Bautista^1,2, Gurdeep Jaswant^1,3,4,5, Hollie French^1,6, Kathryn Campbell¹, Rowan Durrant¹, Robert Gifford^1,6, Grace S. N. Kia^7,8, Brian Ogoti^3,9, Katie Hampson¹, Kirstyn Brunker^1,6

¹School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health, Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

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כאן, אנו מציגים זרימת עבודה מהירה וחסכונית לאפיון גנומים של נגיף הכלבת (RABV) באמצעות טכנולוגיית ננו-נקבוביות. זרימת העבודה נועדה לתמוך במעקב מבוסס גנומיקה ברמה המקומית, לספק מידע על שושלות RABV מסתובבות ומיקומם בתוך פילוגניות אזוריות כדי להנחות אמצעים להדברת כלבת.

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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

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