A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

August 18th, 2023

A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings (Video) | JoVE

02:47 min

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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE

03:08 min

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Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome (Video) | JoVE

05:00 min

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Criselda Bautista^1,2, Gurdeep Jaswant^1,3,4,5, Hollie French^1,6, Kathryn Campbell¹, Rowan Durrant¹, Robert Gifford^1,6, Grace S. N. Kia^7,8, Brian Ogoti^3,9, Katie Hampson¹, Kirstyn Brunker^1,6

¹School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health, Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

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Burada, nanopor teknolojisini kullanarak kuduz virüsü (RABV) genomlarını karakterize etmek için hızlı ve uygun maliyetli bir iş akışı sunuyoruz. İş akışı, kuduz kontrol önlemlerine rehberlik etmek için dolaşımdaki RABV soyları ve bunların bölgesel filogeniler içindeki yerleşimleri hakkında bilgi sağlayarak yerel düzeyde genomik bilgili sürveyansı desteklemeyi amaçlamaktadır.

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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

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