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Advancements in Cultivating Mouse Hair Cells for Auditory Research (Video) | JoVE
Dissection and Removal of the Temporal Bone to Collect Auditory Epithelia from Newborn Mice (Video) | JoVE
Enzymatic Disaggregation of the Auditory Epithelium to Obtain Auditory Hair Cells (Video) | JoVE
在此,我们提出了从小鼠中分离和培养原代耳蜗毛细胞的详细方案。最初,在显微镜下从新生儿(3-5天龄)鼠耳蜗中解剖Corti的器官。随后,将细胞酶消化成单细胞悬液,并在培养数天后使用免疫荧光进行鉴定。
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