Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

December 1st, 2023

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Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center, Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, ³Research Center of Genetic Resources, National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

* These authors contributed equally

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Een langdurige conserveringsmethode voor Drosophila-stammen als alternatief voor de frequente overdracht van volwassen vliegen naar flesjes met vers voedsel is zeer wenselijk. Dit protocol beschrijft de cryopreservatie van Drosophila primordiale kiemcellen en de heropleving van stammen via hun transplantatie naar agametische gastheerembryo's.

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Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

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