Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

December 1st, 2023

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Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center, Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, ³Research Center of Genetic Resources, National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

* These authors contributed equally

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En långsiktig konserveringsmetod för Drosophila-stammar som ett alternativ till den frekventa överföringen av vuxna flugor till färskfoderflaskor är mycket önskvärd. Detta protokoll beskriver kryokonservering av Drosophila primordiala könsceller och stamåterupplivning via deras transplantation till agametiska värdembryon.

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Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

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